Förster resonance energy transfer (FRET)-based small-molecule sensors and imaging agents

Luling Wu, Chusen Huang, Ben P. Emery, Adam C. Sedgwick, Steven D. Bull, Xiao Peng He, He Tian, Juyoung Yoon, Jonathan L. Sessler, Tony D. James

Research output: Contribution to journalReview articlepeer-review

512 Scopus citations


In this tutorial review, we will explore recent advances in the construction and application of Förster resonance energy transfer (FRET)-based small-molecule fluorescent probes. The advantages of FRET-based fluorescent probes include: a large Stokes shift, ratiometric sensing and dual/multi-analyte responsive systems. We discuss the underlying energy donor-acceptor dye combinations and emphasise their applications for the detection or imaging of cations, anions, small neutral molecules, biomacromolecules, cellular microenvionments and dual/multi-analyte responsive systems.

Original languageEnglish
Pages (from-to)5110-5139
Number of pages30
JournalChemical Society Reviews
Issue number15
StatePublished - 7 Aug 2020

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© The Royal Society of Chemistry.


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