Extremely low frequency magnetic fields do not elicit oxidativestress in MCF10A cells

Mi Na Hong; Na Kyung Han; Hyung Chul Lee; Young Kyu Ko; Sung Gil Chi; Yun Sil Lee; Yoon Myung Gimm; Sung Ho Myung; Jae Seon Lee

doi:10.1269/jrr.11049

Extremely low frequency magnetic fields do not elicit oxidativestress in MCF10A cells

Mi Na Hong, Na Kyung Han, Hyung Chul Lee, Young Kyu Ko, Sung Gil Chi, Yun Sil Lee, Yoon Myung Gimm, Sung Ho Myung, Jae Seon Lee

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The aim of this study was to determine whether extremely low frequency magnetic fields (ELF-MF) could affect intracellular reactive oxygen species (ROS) levels and antioxidant enzyme activity. After MCF10A human breast epithelial cells were exposed to 1 mT of 60 Hz ELF-MF for 4 hours, intracellular ROS level, superoxide dismutase (SOD) activity, and reduced to oxidized glutathione (GSH/GSSG) ratio were measured. The cells exposed to ELF-MF did not evidence statistically significant changes in the above-mentioned biological parameters as compared to either the incubator controls or sham-exposed cells. By way of contrast, the IR-exposed cells exhibited marked changes in ROS level, SOD activity, and GSH/GSSG ratio. When we assessed morphological changes and senescence-associated beta-galactosidase (SA-β-Gal) activity, only the IR-exposed cells were positive. According to our results, it could be concluded that ELF-MF has no effect on intracellular ROS level, SOD activity, and GSH/GSSG ratio under our exposure condition.

Original language	English
Pages (from-to)	79-86
Number of pages	8
Journal	Journal of Radiation Research
Volume	53
Issue number	1
DOIs	https://doi.org/10.1269/jrr.11049
State	Published - 2012

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title = "Extremely low frequency magnetic fields do not elicit oxidativestress in MCF10A cells",

abstract = "The aim of this study was to determine whether extremely low frequency magnetic fields (ELF-MF) could affect intracellular reactive oxygen species (ROS) levels and antioxidant enzyme activity. After MCF10A human breast epithelial cells were exposed to 1 mT of 60 Hz ELF-MF for 4 hours, intracellular ROS level, superoxide dismutase (SOD) activity, and reduced to oxidized glutathione (GSH/GSSG) ratio were measured. The cells exposed to ELF-MF did not evidence statistically significant changes in the above-mentioned biological parameters as compared to either the incubator controls or sham-exposed cells. By way of contrast, the IR-exposed cells exhibited marked changes in ROS level, SOD activity, and GSH/GSSG ratio. When we assessed morphological changes and senescence-associated beta-galactosidase (SA-β-Gal) activity, only the IR-exposed cells were positive. According to our results, it could be concluded that ELF-MF has no effect on intracellular ROS level, SOD activity, and GSH/GSSG ratio under our exposure condition.",

author = "Hong, {Mi Na} and Han, {Na Kyung} and Lee, {Hyung Chul} and Ko, {Young Kyu} and Chi, {Sung Gil} and Lee, {Yun Sil} and Gimm, {Yoon Myung} and Myung, {Sung Ho} and Lee, {Jae Seon}",

year = "2012",

doi = "10.1269/jrr.11049",

language = "English",

volume = "53",

pages = "79--86",

journal = "Journal of Radiation Research",

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T1 - Extremely low frequency magnetic fields do not elicit oxidativestress in MCF10A cells

AU - Hong, Mi Na

AU - Han, Na Kyung

AU - Lee, Hyung Chul

AU - Ko, Young Kyu

AU - Chi, Sung Gil

AU - Lee, Yun Sil

AU - Gimm, Yoon Myung

AU - Myung, Sung Ho

AU - Lee, Jae Seon

PY - 2012

Y1 - 2012

N2 - The aim of this study was to determine whether extremely low frequency magnetic fields (ELF-MF) could affect intracellular reactive oxygen species (ROS) levels and antioxidant enzyme activity. After MCF10A human breast epithelial cells were exposed to 1 mT of 60 Hz ELF-MF for 4 hours, intracellular ROS level, superoxide dismutase (SOD) activity, and reduced to oxidized glutathione (GSH/GSSG) ratio were measured. The cells exposed to ELF-MF did not evidence statistically significant changes in the above-mentioned biological parameters as compared to either the incubator controls or sham-exposed cells. By way of contrast, the IR-exposed cells exhibited marked changes in ROS level, SOD activity, and GSH/GSSG ratio. When we assessed morphological changes and senescence-associated beta-galactosidase (SA-β-Gal) activity, only the IR-exposed cells were positive. According to our results, it could be concluded that ELF-MF has no effect on intracellular ROS level, SOD activity, and GSH/GSSG ratio under our exposure condition.

AB - The aim of this study was to determine whether extremely low frequency magnetic fields (ELF-MF) could affect intracellular reactive oxygen species (ROS) levels and antioxidant enzyme activity. After MCF10A human breast epithelial cells were exposed to 1 mT of 60 Hz ELF-MF for 4 hours, intracellular ROS level, superoxide dismutase (SOD) activity, and reduced to oxidized glutathione (GSH/GSSG) ratio were measured. The cells exposed to ELF-MF did not evidence statistically significant changes in the above-mentioned biological parameters as compared to either the incubator controls or sham-exposed cells. By way of contrast, the IR-exposed cells exhibited marked changes in ROS level, SOD activity, and GSH/GSSG ratio. When we assessed morphological changes and senescence-associated beta-galactosidase (SA-β-Gal) activity, only the IR-exposed cells were positive. According to our results, it could be concluded that ELF-MF has no effect on intracellular ROS level, SOD activity, and GSH/GSSG ratio under our exposure condition.

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SN - 0449-3060

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JO - Journal of Radiation Research

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Extremely low frequency magnetic fields do not elicit oxidativestress in MCF10A cells

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