Abstract
Although various marine ingredients have been exploited for the development of cosmetic products, no previous study has examined the potential of seaweed extracellular vesicles (EV) in such applications. Our results revealed that EV from Codium fragile and Sargassum fusiforme effectively decreased α-MSH-mediated melanin synthesis in MNT-1 human melanoma cells, associated with downregulation of MITF (microphthalmia-associated transcription factor), tyrosinase and TRP1 (tyrosinase-related proteins 1). The most effective inhibitory concentrations of EV were 250 μg/ml for S. fusiforme and 25 μg/ml for C. fragile, without affecting the viability of MNT-1 cells. Both EV reduced melanin synthesis in the epidermal basal layer of a three-dimensional model of human epidermis. Moreover, the application of the prototype cream containing C. fragile EV (final 5 μg/ml) yielded 1.31% improvement in skin brightness in a clinical trial. Together, these results suggest that EV from C. fragile and S. fusiforme reduce melanin synthesis and may be potential therapeutic and/or supplementary whitening agents.
Original language | English |
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Pages (from-to) | 736-745 |
Number of pages | 10 |
Journal | Molecules and Cells |
Volume | 44 |
Issue number | 10 |
DOIs | |
State | Published - 31 Oct 2021 |
Bibliographical note
Funding Information:This work was supported by the project titled ‘development of skin functional material using seaweed extracellular vesicle’, funded by the Ministry of Oceans and Fisheries, Korea (20190067). We thank the electron microscopy core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea, for support and instrumentation. This electron microscopy was performed by the core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea.
Funding Information:
This work was supported by the project titled ?development of skin functional material using seaweed extracellular vesicle?, funded by the Ministry of Oceans and Fisheries, Korea (20190067). We thank the electron microscopy core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea, for support and instrumentation. This electron microscopy was performed by the core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea. Written informed consent was obtained from the participants for publication of this article and accompanying images.
Publisher Copyright:
© 2021, Korean Society for Molecular and Cellular Biology. All rights reserved.
Keywords
- Extracellular vesicles
- Melanin synthesis
- Seaweed
- Skin epidermis