Extracellular vesicles from korean codium fragile and sargassum fusiforme negatively regulate melanin synthesis

Bohee Jang; Heesung Chung; Hyejung Jung; Hyun Kuk Song; Eunhye Park; Hack Sun Choi; Kyuhyun Jung; Han Choe; Sanghwa Yang; Eok Soo Oh

doi:10.14348/MOLCELLS.2021.2167

Extracellular vesicles from korean codium fragile and sargassum fusiforme negatively regulate melanin synthesis

Bohee Jang, Heesung Chung, Hyejung Jung, Hyun Kuk Song, Eunhye Park, Hack Sun Choi, Kyuhyun Jung, Han Choe, Sanghwa Yang, Eok Soo Oh

Life Sciences

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Although various marine ingredients have been exploited for the development of cosmetic products, no previous study has examined the potential of seaweed extracellular vesicles (EV) in such applications. Our results revealed that EV from Codium fragile and Sargassum fusiforme effectively decreased α-MSH-mediated melanin synthesis in MNT-1 human melanoma cells, associated with downregulation of MITF (microphthalmia-associated transcription factor), tyrosinase and TRP1 (tyrosinase-related proteins 1). The most effective inhibitory concentrations of EV were 250 μg/ml for S. fusiforme and 25 μg/ml for C. fragile, without affecting the viability of MNT-1 cells. Both EV reduced melanin synthesis in the epidermal basal layer of a three-dimensional model of human epidermis. Moreover, the application of the prototype cream containing C. fragile EV (final 5 μg/ml) yielded 1.31% improvement in skin brightness in a clinical trial. Together, these results suggest that EV from C. fragile and S. fusiforme reduce melanin synthesis and may be potential therapeutic and/or supplementary whitening agents.

Original language	English
Pages (from-to)	736-745
Number of pages	10
Journal	Molecules and Cells
Volume	44
Issue number	10
DOIs	https://doi.org/10.14348/MOLCELLS.2021.2167
State	Published - 31 Oct 2021

Bibliographical note

Funding Information:
This work was supported by the project titled ‘development of skin functional material using seaweed extracellular vesicle’, funded by the Ministry of Oceans and Fisheries, Korea (20190067). We thank the electron microscopy core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea, for support and instrumentation. This electron microscopy was performed by the core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea.

Funding Information:
This work was supported by the project titled ?development of skin functional material using seaweed extracellular vesicle?, funded by the Ministry of Oceans and Fisheries, Korea (20190067). We thank the electron microscopy core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea, for support and instrumentation. This electron microscopy was performed by the core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea. Written informed consent was obtained from the participants for publication of this article and accompanying images.

Publisher Copyright:
© 2021, Korean Society for Molecular and Cellular Biology. All rights reserved.

Keywords

Extracellular vesicles
Melanin synthesis
Seaweed
Skin epidermis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.14348/MOLCELLS.2021.2167

Cite this

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title = "Extracellular vesicles from korean codium fragile and sargassum fusiforme negatively regulate melanin synthesis",

abstract = "Although various marine ingredients have been exploited for the development of cosmetic products, no previous study has examined the potential of seaweed extracellular vesicles (EV) in such applications. Our results revealed that EV from Codium fragile and Sargassum fusiforme effectively decreased α-MSH-mediated melanin synthesis in MNT-1 human melanoma cells, associated with downregulation of MITF (microphthalmia-associated transcription factor), tyrosinase and TRP1 (tyrosinase-related proteins 1). The most effective inhibitory concentrations of EV were 250 μg/ml for S. fusiforme and 25 μg/ml for C. fragile, without affecting the viability of MNT-1 cells. Both EV reduced melanin synthesis in the epidermal basal layer of a three-dimensional model of human epidermis. Moreover, the application of the prototype cream containing C. fragile EV (final 5 μg/ml) yielded 1.31% improvement in skin brightness in a clinical trial. Together, these results suggest that EV from C. fragile and S. fusiforme reduce melanin synthesis and may be potential therapeutic and/or supplementary whitening agents.",

keywords = "Extracellular vesicles, Melanin synthesis, Seaweed, Skin epidermis",

author = "Bohee Jang and Heesung Chung and Hyejung Jung and Song, {Hyun Kuk} and Eunhye Park and Choi, {Hack Sun} and Kyuhyun Jung and Han Choe and Sanghwa Yang and Oh, {Eok Soo}",

note = "Funding Information: This work was supported by the project titled {\textquoteleft}development of skin functional material using seaweed extracellular vesicle{\textquoteright}, funded by the Ministry of Oceans and Fisheries, Korea (20190067). We thank the electron microscopy core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea, for support and instrumentation. This electron microscopy was performed by the core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea. Funding Information: This work was supported by the project titled ?development of skin functional material using seaweed extracellular vesicle?, funded by the Ministry of Oceans and Fisheries, Korea (20190067). We thank the electron microscopy core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea, for support and instrumentation. This electron microscopy was performed by the core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea. Written informed consent was obtained from the participants for publication of this article and accompanying images. Publisher Copyright: {\textcopyright} 2021, Korean Society for Molecular and Cellular Biology. All rights reserved.",

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AU - Jang, Bohee

AU - Chung, Heesung

AU - Jung, Hyejung

AU - Song, Hyun Kuk

AU - Park, Eunhye

AU - Choi, Hack Sun

AU - Jung, Kyuhyun

AU - Choe, Han

AU - Yang, Sanghwa

AU - Oh, Eok Soo

N1 - Funding Information: This work was supported by the project titled ‘development of skin functional material using seaweed extracellular vesicle’, funded by the Ministry of Oceans and Fisheries, Korea (20190067). We thank the electron microscopy core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea, for support and instrumentation. This electron microscopy was performed by the core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea. Funding Information: This work was supported by the project titled ?development of skin functional material using seaweed extracellular vesicle?, funded by the Ministry of Oceans and Fisheries, Korea (20190067). We thank the electron microscopy core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea, for support and instrumentation. This electron microscopy was performed by the core facility at the ConveRgence mEDIcine research cenTer (CREDIT), Asan Medical Center, Seoul, Korea. Written informed consent was obtained from the participants for publication of this article and accompanying images. Publisher Copyright: © 2021, Korean Society for Molecular and Cellular Biology. All rights reserved.

PY - 2021/10/31

Y1 - 2021/10/31

N2 - Although various marine ingredients have been exploited for the development of cosmetic products, no previous study has examined the potential of seaweed extracellular vesicles (EV) in such applications. Our results revealed that EV from Codium fragile and Sargassum fusiforme effectively decreased α-MSH-mediated melanin synthesis in MNT-1 human melanoma cells, associated with downregulation of MITF (microphthalmia-associated transcription factor), tyrosinase and TRP1 (tyrosinase-related proteins 1). The most effective inhibitory concentrations of EV were 250 μg/ml for S. fusiforme and 25 μg/ml for C. fragile, without affecting the viability of MNT-1 cells. Both EV reduced melanin synthesis in the epidermal basal layer of a three-dimensional model of human epidermis. Moreover, the application of the prototype cream containing C. fragile EV (final 5 μg/ml) yielded 1.31% improvement in skin brightness in a clinical trial. Together, these results suggest that EV from C. fragile and S. fusiforme reduce melanin synthesis and may be potential therapeutic and/or supplementary whitening agents.

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Extracellular vesicles from korean codium fragile and sargassum fusiforme negatively regulate melanin synthesis

Abstract

Bibliographical note

Keywords

UN SDGs

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