Expression, solubilization and purification of a human olfactory receptor from escherichia coli

Hyun Seok Song, Sang Hun Lee, Eun Hae Oh, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Olfactory receptors pertaining to G protein-coupled receptor (GPCR) are integral membrane proteins composed of seven transmembrane spanning domains. It has been reported that these receptor proteins are difficult to overexpress, solubilize, and purify because of their complicated structures and strong hydrophobicity. In this study, full-length human olfactory receptor (hOR) 2AG1 was overexpressed in E. coli as a fusion protein with a glutathione S-transferase (GST) tag mainly as an inclusion body without any mutations or deletions in the gene. This protein was difficult to solubilize with detergents and chaotropic agents, and only N-lauroyl sarcosine was found to be suitable for solubilizing it. In contrast, Trition X-100 was found to solubilize most of the impurity proteins from the insoluble fraction in E. coli. Based on this observation, we applied a simple and efficient column-free method using these two detergents for the purification of the olfactory receptor protein. In this method, the insoluble fraction of the cell lysate was first treated with Triton X-100 to remove impurity proteins. The remaining insoluble fraction was then further treated with N-lauroyl sarcosine to solubilize the olfactory receptor protein. Milligram quantity of the human olfactory receptor was produced. This is the first report to produce full-length of the olfactory receptor from E. coli.

Original languageEnglish
Pages (from-to)309-314
Number of pages6
JournalCurrent Microbiology
Volume59
Issue number3
DOIs
StatePublished - Sep 2009

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF ) grant funded by the Korea government (MEST) (No. 2009-0080242). This work was also partially supported by the System 2010 program of the Ministry of Knowledge Economy.

Fingerprint

Dive into the research topics of 'Expression, solubilization and purification of a human olfactory receptor from escherichia coli'. Together they form a unique fingerprint.

Cite this