Expression, purification and crystallization of recombinant human TRAIL

Sun Shin Cha, Hang Cheol Shin, Kwan Yong Choi, Byung Ha Oh

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


TRAIL (also known as Apo-2L) belongs to the tumour necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumour cell lines upon binding to the death-signalling receptors on the cell membrane. Normal cells are resistant to TRAIL, owing to the expression of decoy receptors which lack functional death domains and antagonize TRAIL-induced apoptosis. Soluble and functional human TRAIL, expressed in Escherichia coli and refolded into a functional form, has been crystallized. The crystals belong to space group P63 with unit-cell dimensions a = b = 65.61, c = 131.70 Å. The asymmetric unit contains two molecules of TRAIL, with a crystal volume per protein mass (V(m)) of 2.41 Å3 Da-1 and a solvent content of about 42% by volume. A native and a platinum-derivative data set to 2.8 and 3.5 Å resolution, respectively, were obtained from frozen crystals. Structure determination by a combined molecular replacement and isomorphous replacement method is in progress.

Original languageEnglish
Pages (from-to)1101-1104
Number of pages4
JournalActa Crystallographica Section D: Biological Crystallography
Issue number5
StatePublished - May 1999


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