Expression of dihydropyridine-sensitive brain calcium channels in the rat central nervous system

Hemin Chin, Mark A. Smith, Hyung Lae Kim, Hyun Kim

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


We have localized dihydropyridine (DHP)-sensitive calcium channels in rat brain by in situ hybridization and immunohistochemistry. The mRNA for the dihydropyridine-sensitive calcium channel α1 subunit (DHPR-B) is prominently localized in neuronal cells in the olfactory bulb, dentate gyrus, hippocampus, arcuate nucleus, paraventricular nucleus, ventromedial nucleus, cerebral cortex, superior colliculus and the cerebellar Purkinje cell layer. Strong expression of DHPR-B mRNA was also found in the pituitary and pineal glands. DHP-sensitive calcium channel α1 subunit distribution has also been examined immunohistochemically with polyclonal antibodies raised against synthetic peptides specific for the DHPR-B α1 subunit protein. The results from immunohistochemistry were in good agreement with those from in situ hybridization. Thus, regional distribution and localization of DHPR-B mRNA and α1 subunit protein in rat brain suggest that this type of DHP-sensitive brain calcium channel may play an important role in excitation-secretion coupling functions in the neuroendocrine system.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalFEBS Letters
Issue number1
StatePublished - 2 Mar 1992


  • Brain
  • Ca-channel
  • Immunohistochemistry
  • in situ hybridization


Dive into the research topics of 'Expression of dihydropyridine-sensitive brain calcium channels in the rat central nervous system'. Together they form a unique fingerprint.

Cite this