Expression of ammonia transporters, Rhbg and Rhcg, in chronic cyclosporine nephropathy in rats

Sun Woo Lim, Kyung Ohk Ahn, Wan Young Kim, Dong He Han, Can Li, Jung Yeon Ghee, Ki Hwan Han, Hye Young Kim, Mary E. Handlogten, Jin Kim, Chul Woo Yang, I. David Weiner

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Background/Aims: Cyclosporine (CsA)-induced renal injury causes renal tubular acidosis. The current study was performed to evaluate the influence of CsA-induced renal injury on the ammonia transporter family members, Rh B-glycoprotein (Rhbg) and Rh C-glycoprotein (Rhcg). Methods: Rats were treated daily for 1 or 4 weeks with vehicle (VH) or CsA. Induction of chronic CsA-induced nephropathy was confirmed by demonstrating impaired renal function and characteristic histopathology. Rhbg and Rhcg expression was evaluated with immunoblot, immunohistochemistry, real-time RT-PCR and electron microscopy. Results: CsA treatment for 4 weeks developed mild metabolic acidosis and decreased urinary ammonia excretion. Rhcg mRNA expression was unchanged in both the cortex and outer medulla, but Rhcg protein expression in the CsA group was significantly reduced in the cortex and outer medulla. There were no significant differences in Rhbg mRNA and protein expression between the CsA and VH group. Conclusion: Long-term treatment with CsA in rats results in decreased urinary ammonia excretion accompanied by decreased expression of Rhcg; these changes are likely to mediate the CsA-induced defect in ammonium excretion in the collecting duct.

Original languageEnglish
Pages (from-to)e49-e58
JournalNephron - Experimental Nephrology
Volume110
Issue number2
DOIs
StatePublished - Nov 2008

Keywords

  • Ammonia transporter
  • Chronic cyclosporine nephropathy
  • Kidney

Fingerprint

Dive into the research topics of 'Expression of ammonia transporters, Rhbg and Rhcg, in chronic cyclosporine nephropathy in rats'. Together they form a unique fingerprint.

Cite this