TY - JOUR
T1 - Expression, crystallization and preliminary X-ray crystallographic analysis of d-alanine-d-alanine ligase from OXA-23-producing Acinetobacter baumannii K0420859
AU - Huynh, Kim Hung
AU - Tran, Huyen Thi
AU - Pham, Tan Viet
AU - Ngo, Ho Phuong Thuy
AU - Cha, Sun Shin
AU - Chung, Kyung Min
AU - Lee, Sang Hee
AU - Kang, Lin Woo
PY - 2014/4
Y1 - 2014/4
N2 - Acinetobacter baumannii causes bacteraemia, pneumonia, other respiratory-tract and urinary-tract infections in humans. OXA-23 carbapenemase-producing A. baumannii K0420859 (A. baumannii OXA-23) is resistant to carbapenem, a common antibacterial drug. To develop an efficient and novel antibacterial drug against A. baumannii OXA-23, d-alanine-d-alanine ligase, which is essential in bacterial cell-wall synthesis, is of interest. Here, the d-alanine-d-alanine ligase (AbDdl) gene from A. baumannii OXA-23 was cloned and expressed, and the AbDdl protein was purified and crystallized; this enzyme can be used as a novel target for an antibacterial drug against A. baumannii OXA-23. The AbDdl crystal diffracted to a resolution of 2.8 Å and belonged to the orthorhombic space group P212121, with unit-cell parameters a = 113.4, b = 116.7, c = 176.5 Å, a corresponding V M of 2.8 Å3 Da -1 and a solvent content of 56.3%, and six protomers in the asymmetric unit.
AB - Acinetobacter baumannii causes bacteraemia, pneumonia, other respiratory-tract and urinary-tract infections in humans. OXA-23 carbapenemase-producing A. baumannii K0420859 (A. baumannii OXA-23) is resistant to carbapenem, a common antibacterial drug. To develop an efficient and novel antibacterial drug against A. baumannii OXA-23, d-alanine-d-alanine ligase, which is essential in bacterial cell-wall synthesis, is of interest. Here, the d-alanine-d-alanine ligase (AbDdl) gene from A. baumannii OXA-23 was cloned and expressed, and the AbDdl protein was purified and crystallized; this enzyme can be used as a novel target for an antibacterial drug against A. baumannii OXA-23. The AbDdl crystal diffracted to a resolution of 2.8 Å and belonged to the orthorhombic space group P212121, with unit-cell parameters a = 113.4, b = 116.7, c = 176.5 Å, a corresponding V M of 2.8 Å3 Da -1 and a solvent content of 56.3%, and six protomers in the asymmetric unit.
KW - OXA-23-producing Acinetobacter baumannii
KW - cell-wall synthesis
KW - d-alanine-d-alanine ligase
UR - http://www.scopus.com/inward/record.url?scp=84905513478&partnerID=8YFLogxK
U2 - 10.1107/S2053230X14005081
DO - 10.1107/S2053230X14005081
M3 - Article
C2 - 24699750
AN - SCOPUS:84905513478
SN - 2053-230X
VL - 70
SP - 505
EP - 508
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
IS - 4
ER -