Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli

Irisappan Ganesh; Sambandam Ravikumar; Si Jae Park; Seung Hwan Lee; Soon Ho Hong

doi:10.1007/s11814-013-0061-4

Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli

Irisappan Ganesh, Sambandam Ravikumar, Si Jae Park, Seung Hwan Lee, Soon Ho Hong

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The malate-pyruvate conversion pathway is catalyzed by two malic enzyme isomers, MaeA and MaeB. qRT-PCR was carried out under malate and pyruvate supplemented conditions to understand the dynamics of maeA and maeB gene expression. maeA expression was elevated by malate, and maeB expression was inhibited by levels of both malate and pyruvate higher than 0.1 mM. Green fluorescent protein (GFP) reporter plasmids were also constructed by integration of the maeA/maeB promoter with the gfp gene. We showed that maeA driven GFP expression was positively and negatively correlated with extracellular malate and pyruvate induction. In contrast, no significant changes in maeB driven GFP expression were observed under both malate and pyruvate supplemented conditions.

Original language	English
Pages (from-to)	1443-1447
Number of pages	5
Journal	Korean Journal of Chemical Engineering
Volume	30
Issue number	7
DOIs	https://doi.org/10.1007/s11814-013-0061-4
State	Published - Jul 2013

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0022392).

Keywords

Escherichia coli
Green Fluorescent Protein
Malate
Pyruvate
maeA
maeB

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title = "Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli",

abstract = "The malate-pyruvate conversion pathway is catalyzed by two malic enzyme isomers, MaeA and MaeB. qRT-PCR was carried out under malate and pyruvate supplemented conditions to understand the dynamics of maeA and maeB gene expression. maeA expression was elevated by malate, and maeB expression was inhibited by levels of both malate and pyruvate higher than 0.1 mM. Green fluorescent protein (GFP) reporter plasmids were also constructed by integration of the maeA/maeB promoter with the gfp gene. We showed that maeA driven GFP expression was positively and negatively correlated with extracellular malate and pyruvate induction. In contrast, no significant changes in maeB driven GFP expression were observed under both malate and pyruvate supplemented conditions.",

keywords = "Escherichia coli, Green Fluorescent Protein, Malate, Pyruvate, maeA, maeB",

author = "Irisappan Ganesh and Sambandam Ravikumar and Park, {Si Jae} and Lee, {Seung Hwan} and Hong, {Soon Ho}",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0022392).",

year = "2013",

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doi = "10.1007/s11814-013-0061-4",

language = "English",

volume = "30",

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T1 - Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli

AU - Ganesh, Irisappan

AU - Ravikumar, Sambandam

AU - Park, Si Jae

AU - Lee, Seung Hwan

AU - Hong, Soon Ho

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0022392).

PY - 2013/7

Y1 - 2013/7

N2 - The malate-pyruvate conversion pathway is catalyzed by two malic enzyme isomers, MaeA and MaeB. qRT-PCR was carried out under malate and pyruvate supplemented conditions to understand the dynamics of maeA and maeB gene expression. maeA expression was elevated by malate, and maeB expression was inhibited by levels of both malate and pyruvate higher than 0.1 mM. Green fluorescent protein (GFP) reporter plasmids were also constructed by integration of the maeA/maeB promoter with the gfp gene. We showed that maeA driven GFP expression was positively and negatively correlated with extracellular malate and pyruvate induction. In contrast, no significant changes in maeB driven GFP expression were observed under both malate and pyruvate supplemented conditions.

AB - The malate-pyruvate conversion pathway is catalyzed by two malic enzyme isomers, MaeA and MaeB. qRT-PCR was carried out under malate and pyruvate supplemented conditions to understand the dynamics of maeA and maeB gene expression. maeA expression was elevated by malate, and maeB expression was inhibited by levels of both malate and pyruvate higher than 0.1 mM. Green fluorescent protein (GFP) reporter plasmids were also constructed by integration of the maeA/maeB promoter with the gfp gene. We showed that maeA driven GFP expression was positively and negatively correlated with extracellular malate and pyruvate induction. In contrast, no significant changes in maeB driven GFP expression were observed under both malate and pyruvate supplemented conditions.

KW - Escherichia coli

KW - Green Fluorescent Protein

KW - Malate

KW - Pyruvate

KW - maeA

KW - maeB

UR - http://www.scopus.com/inward/record.url?scp=84879844379&partnerID=8YFLogxK

U2 - 10.1007/s11814-013-0061-4

DO - 10.1007/s11814-013-0061-4

M3 - Article

AN - SCOPUS:84879844379

SN - 0256-1115

VL - 30

SP - 1443

EP - 1447

JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

IS - 7

ER -

Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli

Abstract

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Keywords

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