Expression and localization of translationally controlled tumor protein in rat urinary organs

Vadim Sheverdin, Seong Yeon Bae, Dong Hae Shin, Kyunglim Lee

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

This study describes the very first assessment of the expression and localization of translationally controlled tumor protein (TCTP) in adult rat urinary organs. TCTP expression levels in kidneys, urinary bladder, and urethra were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting, and its cellular localization was examined immunohistochemically in paraffin sections of various urinary organs. TCTP was found in all urinary organs. Its expression was high in the urethra and low in the bladder. TCTP was localized in glomerular podocytes, epithelium of proximal and distal renal tubules, in the loop of Henle, and in the transitional epithelium of the bladder and urethra, mostly in basal cell layers). The subcellular localization of TCTP in these urinary organs was cytoplasmic. These findings suggest that TCTP may be involved in urine formation and excretion.

Original languageEnglish
Pages (from-to)1576-1581
Number of pages6
JournalMicroscopy Research and Technique
Volume75
Issue number11
DOIs
StatePublished - Nov 2012

Keywords

  • K-ATPase
  • Kidney
  • Na
  • TCTP
  • Urethra
  • Urinary bladder

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