Exploiting fruit waste grape pomace for silver nanoparticles synthesis, assessing their antioxidant, antidiabetic potential and antibacterial activity against human pathogens: A novel approach

Ganesh Dattatraya Saratale; Rijuta Ganesh Saratale; Dong Su Kim; Do Yeong Kim; Han Seung Shin

doi:10.3390/nano10081457

Exploiting fruit waste grape pomace for silver nanoparticles synthesis, assessing their antioxidant, antidiabetic potential and antibacterial activity against human pathogens: A novel approach

Ganesh Dattatraya Saratale, Rijuta Ganesh Saratale, Dong Su Kim, Do Yeong Kim, Han Seung Shin

Department of Environmental Science & Engineering

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Grape pomace, a most abundant and renewable wine industry waste product was utilized as a suitable reducing, capping, and stabilizing biomolecules for green synthesis of silver nanoparticles (AgNPs). The physicochemical properties of biosynthesized grape pomace extract (GPE)-AgNPs were duly appraised via UV–Visible spectroscopy, X-ray diffractometer (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy. The analytical studies revealed that the GPE-AgNPs were well formed and stable in nature. The functional groups of organic molecules of GPE are present on the surface of AgNPs with average NPs diameter in the range of 20–35 nm. GPE-AgNPs exhibited significant free radical scavenging activity mainly DPPH radical (IC₅₀, 50.0 ± 2.25 μg/mL) and ABTS radical (IC₅₀, 38.46 ± 1.14 μg/mL). Additionally, the synthesized AgNPs showed noticeable inhibition of carbohydrate hydrolyzing enzymes mainly, α-amylase (IC₅₀, 60.2 ± 2.15 μg/mL) and α-glucosidase (IC₅₀, 62.5 ± 2.75 μg/mL). The GPE fabricated AgNPs showed noteworthy antibacterial potential against infectious bacteria viz., Escherichia coli and Staphylococcus aureus. The reaction mechanism of antibacterial activity was studied by measuring the bacterial cell membrane breakage and cytoplasmic contents, mainly, nucleic acid, proteins, and reducing sugar. Therefore, this research attempt illustrated the potential of GPE as a novel source intended for the biosynthesis of AgNPs that may open up new horizons in the field of nanomedicine.

Original language	English
Article number	1457
Pages (from-to)	1-18
Number of pages	18
Journal	Nanomaterials
Volume	10
Issue number	8
DOIs	https://doi.org/10.3390/nano10081457
State	Published - Aug 2020

Bibliographical note

Funding Information:
Acknowledgments: The authors gratefully acknowledge the research support by Dongguk University, South Korea, under research fund 2018–2020. Authors are thankful to Shrikrishna D. Sartale, Savitribai Phule Pune University, Pune, India for his help for the analytical studies.

Funding Information:
This research work was funded by Korea Environmental Industry & Technology Institute: (A117-00197-0703-0).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Antidiabetic activity
Antioxidant potential
Cytoplasmic leakages
Grape pomace
Silver nanoparticles

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10.3390/nano10081457

Cite this

@article{6ef62fb329d647c684c577598cb19e7f,

title = "Exploiting fruit waste grape pomace for silver nanoparticles synthesis, assessing their antioxidant, antidiabetic potential and antibacterial activity against human pathogens: A novel approach",

abstract = "Grape pomace, a most abundant and renewable wine industry waste product was utilized as a suitable reducing, capping, and stabilizing biomolecules for green synthesis of silver nanoparticles (AgNPs). The physicochemical properties of biosynthesized grape pomace extract (GPE)-AgNPs were duly appraised via UV–Visible spectroscopy, X-ray diffractometer (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy. The analytical studies revealed that the GPE-AgNPs were well formed and stable in nature. The functional groups of organic molecules of GPE are present on the surface of AgNPs with average NPs diameter in the range of 20–35 nm. GPE-AgNPs exhibited significant free radical scavenging activity mainly DPPH radical (IC50, 50.0 ± 2.25 μg/mL) and ABTS radical (IC50, 38.46 ± 1.14 μg/mL). Additionally, the synthesized AgNPs showed noticeable inhibition of carbohydrate hydrolyzing enzymes mainly, α-amylase (IC50, 60.2 ± 2.15 μg/mL) and α-glucosidase (IC50, 62.5 ± 2.75 μg/mL). The GPE fabricated AgNPs showed noteworthy antibacterial potential against infectious bacteria viz., Escherichia coli and Staphylococcus aureus. The reaction mechanism of antibacterial activity was studied by measuring the bacterial cell membrane breakage and cytoplasmic contents, mainly, nucleic acid, proteins, and reducing sugar. Therefore, this research attempt illustrated the potential of GPE as a novel source intended for the biosynthesis of AgNPs that may open up new horizons in the field of nanomedicine.",

keywords = "Antidiabetic activity, Antioxidant potential, Cytoplasmic leakages, Grape pomace, Silver nanoparticles",

author = "Saratale, {Ganesh Dattatraya} and Saratale, {Rijuta Ganesh} and Kim, {Dong Su} and Kim, {Do Yeong} and Shin, {Han Seung}",

note = "Funding Information: Acknowledgments: The authors gratefully acknowledge the research support by Dongguk University, South Korea, under research fund 2018–2020. Authors are thankful to Shrikrishna D. Sartale, Savitribai Phule Pune University, Pune, India for his help for the analytical studies. Funding Information: This research work was funded by Korea Environmental Industry & Technology Institute: (A117-00197-0703-0). Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = aug,

doi = "10.3390/nano10081457",

language = "English",

volume = "10",

pages = "1--18",

journal = "Nanomaterials",

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Exploiting fruit waste grape pomace for silver nanoparticles synthesis, assessing their antioxidant, antidiabetic potential and antibacterial activity against human pathogens: A novel approach. / Saratale, Ganesh Dattatraya; Saratale, Rijuta Ganesh; Kim, Dong Su et al.
In: Nanomaterials, Vol. 10, No. 8, 1457, 08.2020, p. 1-18.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Exploiting fruit waste grape pomace for silver nanoparticles synthesis, assessing their antioxidant, antidiabetic potential and antibacterial activity against human pathogens

T2 - A novel approach

AU - Saratale, Ganesh Dattatraya

AU - Saratale, Rijuta Ganesh

AU - Kim, Dong Su

AU - Kim, Do Yeong

AU - Shin, Han Seung

N1 - Funding Information: Acknowledgments: The authors gratefully acknowledge the research support by Dongguk University, South Korea, under research fund 2018–2020. Authors are thankful to Shrikrishna D. Sartale, Savitribai Phule Pune University, Pune, India for his help for the analytical studies. Funding Information: This research work was funded by Korea Environmental Industry & Technology Institute: (A117-00197-0703-0). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/8

Y1 - 2020/8

N2 - Grape pomace, a most abundant and renewable wine industry waste product was utilized as a suitable reducing, capping, and stabilizing biomolecules for green synthesis of silver nanoparticles (AgNPs). The physicochemical properties of biosynthesized grape pomace extract (GPE)-AgNPs were duly appraised via UV–Visible spectroscopy, X-ray diffractometer (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy. The analytical studies revealed that the GPE-AgNPs were well formed and stable in nature. The functional groups of organic molecules of GPE are present on the surface of AgNPs with average NPs diameter in the range of 20–35 nm. GPE-AgNPs exhibited significant free radical scavenging activity mainly DPPH radical (IC50, 50.0 ± 2.25 μg/mL) and ABTS radical (IC50, 38.46 ± 1.14 μg/mL). Additionally, the synthesized AgNPs showed noticeable inhibition of carbohydrate hydrolyzing enzymes mainly, α-amylase (IC50, 60.2 ± 2.15 μg/mL) and α-glucosidase (IC50, 62.5 ± 2.75 μg/mL). The GPE fabricated AgNPs showed noteworthy antibacterial potential against infectious bacteria viz., Escherichia coli and Staphylococcus aureus. The reaction mechanism of antibacterial activity was studied by measuring the bacterial cell membrane breakage and cytoplasmic contents, mainly, nucleic acid, proteins, and reducing sugar. Therefore, this research attempt illustrated the potential of GPE as a novel source intended for the biosynthesis of AgNPs that may open up new horizons in the field of nanomedicine.

AB - Grape pomace, a most abundant and renewable wine industry waste product was utilized as a suitable reducing, capping, and stabilizing biomolecules for green synthesis of silver nanoparticles (AgNPs). The physicochemical properties of biosynthesized grape pomace extract (GPE)-AgNPs were duly appraised via UV–Visible spectroscopy, X-ray diffractometer (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy. The analytical studies revealed that the GPE-AgNPs were well formed and stable in nature. The functional groups of organic molecules of GPE are present on the surface of AgNPs with average NPs diameter in the range of 20–35 nm. GPE-AgNPs exhibited significant free radical scavenging activity mainly DPPH radical (IC50, 50.0 ± 2.25 μg/mL) and ABTS radical (IC50, 38.46 ± 1.14 μg/mL). Additionally, the synthesized AgNPs showed noticeable inhibition of carbohydrate hydrolyzing enzymes mainly, α-amylase (IC50, 60.2 ± 2.15 μg/mL) and α-glucosidase (IC50, 62.5 ± 2.75 μg/mL). The GPE fabricated AgNPs showed noteworthy antibacterial potential against infectious bacteria viz., Escherichia coli and Staphylococcus aureus. The reaction mechanism of antibacterial activity was studied by measuring the bacterial cell membrane breakage and cytoplasmic contents, mainly, nucleic acid, proteins, and reducing sugar. Therefore, this research attempt illustrated the potential of GPE as a novel source intended for the biosynthesis of AgNPs that may open up new horizons in the field of nanomedicine.

KW - Antidiabetic activity

KW - Antioxidant potential

KW - Cytoplasmic leakages

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ER -

Exploiting fruit waste grape pomace for silver nanoparticles synthesis, assessing their antioxidant, antidiabetic potential and antibacterial activity against human pathogens: A novel approach

Abstract

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Keywords

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