Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells

Rijuta Ganesh Saratale; Han Seung Shin; Gopalakrishnan Kumar; Giovanni Benelli; Gajanan S. Ghodake; Yuan Yuan Jiang; Dong Su Kim; Ganesh Dattatraya Saratale

doi:10.1007/s11356-017-8724-z

Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells

Rijuta Ganesh Saratale, Han Seung Shin, Gopalakrishnan Kumar, Giovanni Benelli, Gajanan S. Ghodake, Yuan Yuan Jiang, Dong Su Kim, Ganesh Dattatraya Saratale

Department of Environmental Science & Engineering

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Process byproducts from the fruit industry may represent a cheap and reliable source of green reducing agents to be used in current bio-nanosynthesis. This study reports the use of orange (Citrus × clementina) peel aqueous extract (OPE) for one-pot green synthesis of silver nanoparticles (AgNPs) with high effectiveness against various microbial pathogens as well as rat glial tumor C6 cells. The effects of various operational parameters on the synthesis of AgNPs were systematically investigated. The morphology, particle size, and properties of synthesized AgNPs were characterized using UV–visible spectroscopy, x-ray diffraction, x-ray photoelectron spectroscopy, field emission scanning electron microscopy, energy-dispersive x-ray spectroscopy, and Fourier transform infrared spectroscopy. High-resolution transmission electron microscopy shows that the nanoparticles are mostly spherical in shape and monodispersed, with an average particle size of 15–20 nm. Notably, the OPE-synthesized AgNPs were stable up to 6 months without change in their properties. Low doses of OPE-AgNPs inhibited the growth of human pathogens Escherichia coli, Bacillus cereus, and Staphylococcus aureus. The minimum inhibitory concentration and minimum bactericidal concentration of AgNPs against selected pathogenic bacteria were determined. OPE-AgNPs exhibited strong antioxidant activity in terms of ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging (IC₅₀ 49.6 μg/mL) and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging (IC₅₀ 63.4 μg/mL). OPE-AgNPs showed dose-dependent response against rat glial tumor C6 cells (LD₅₀ 60 μg/mL) showing a promising potential as anticancer agents. Overall, the current investigation highlighted a cheap green technology route to synthesize AgNPs using OPE byproducts and could potentially be utilized in biomedical, cosmetic, and pharmaceutical industry.

Original language	English
Pages (from-to)	10250-10263
Number of pages	14
Journal	Environmental Science and Pollution Research
Volume	25
Issue number	11
DOIs	https://doi.org/10.1007/s11356-017-8724-z
State	Published - 1 Apr 2018

Bibliographical note

Funding Information:
This research was supported by the Agricultural Research Center funded by the Ministry of Food, Forestry, and Fisheries, Korea. One of the author GK acknowledges Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (Grant No: 2016H1D3A1908953). The authors would like to thank Prof. Shrikrishna D. Sartale, Department of Physics, Savitribai Phule Pune University, India, for availing the HR-TEM facility. The authors are also thankful to Mr. Oh Sung-Taek, Dongguk University, for his technical help during anticancer studies.

Publisher Copyright:
© 2017, Springer-Verlag Berlin Heidelberg.

Keywords

ABTS and DPPH free radical scavenging activity
Antimicrobial activity
Green synthesis
HR-TEM
In vitro cytotoxicity
Orange peel extract

Access to Document

10.1007/s11356-017-8724-z

Fingerprint

Dive into the research topics of 'Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells'. Together they form a unique fingerprint.

Cite this

Saratale, R. G., Shin, H. S., Kumar, G., Benelli, G., Ghodake, G. S., Jiang, Y. Y., Kim, D. S., & Saratale, G. D. (2018). Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells. Environmental Science and Pollution Research, 25(11), 10250-10263. https://doi.org/10.1007/s11356-017-8724-z

Saratale, Rijuta Ganesh ; Shin, Han Seung ; Kumar, Gopalakrishnan et al. / Exploiting fruit byproducts for eco-friendly nanosynthesis : Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells. In: Environmental Science and Pollution Research. 2018 ; Vol. 25, No. 11. pp. 10250-10263.

@article{46e6c02c9a65484b9789bf1a47e2f0c9,

title = "Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells",

abstract = "Process byproducts from the fruit industry may represent a cheap and reliable source of green reducing agents to be used in current bio-nanosynthesis. This study reports the use of orange (Citrus × clementina) peel aqueous extract (OPE) for one-pot green synthesis of silver nanoparticles (AgNPs) with high effectiveness against various microbial pathogens as well as rat glial tumor C6 cells. The effects of various operational parameters on the synthesis of AgNPs were systematically investigated. The morphology, particle size, and properties of synthesized AgNPs were characterized using UV–visible spectroscopy, x-ray diffraction, x-ray photoelectron spectroscopy, field emission scanning electron microscopy, energy-dispersive x-ray spectroscopy, and Fourier transform infrared spectroscopy. High-resolution transmission electron microscopy shows that the nanoparticles are mostly spherical in shape and monodispersed, with an average particle size of 15–20 nm. Notably, the OPE-synthesized AgNPs were stable up to 6 months without change in their properties. Low doses of OPE-AgNPs inhibited the growth of human pathogens Escherichia coli, Bacillus cereus, and Staphylococcus aureus. The minimum inhibitory concentration and minimum bactericidal concentration of AgNPs against selected pathogenic bacteria were determined. OPE-AgNPs exhibited strong antioxidant activity in terms of ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging (IC50 49.6 μg/mL) and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging (IC50 63.4 μg/mL). OPE-AgNPs showed dose-dependent response against rat glial tumor C6 cells (LD50 60 μg/mL) showing a promising potential as anticancer agents. Overall, the current investigation highlighted a cheap green technology route to synthesize AgNPs using OPE byproducts and could potentially be utilized in biomedical, cosmetic, and pharmaceutical industry.",

keywords = "ABTS and DPPH free radical scavenging activity, Antimicrobial activity, Green synthesis, HR-TEM, In vitro cytotoxicity, Orange peel extract",

author = "Saratale, {Rijuta Ganesh} and Shin, {Han Seung} and Gopalakrishnan Kumar and Giovanni Benelli and Ghodake, {Gajanan S.} and Jiang, {Yuan Yuan} and Kim, {Dong Su} and Saratale, {Ganesh Dattatraya}",

note = "Funding Information: This research was supported by the Agricultural Research Center funded by the Ministry of Food, Forestry, and Fisheries, Korea. One of the author GK acknowledges Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (Grant No: 2016H1D3A1908953). The authors would like to thank Prof. Shrikrishna D. Sartale, Department of Physics, Savitribai Phule Pune University, India, for availing the HR-TEM facility. The authors are also thankful to Mr. Oh Sung-Taek, Dongguk University, for his technical help during anticancer studies. Publisher Copyright: {\textcopyright} 2017, Springer-Verlag Berlin Heidelberg.",

year = "2018",

month = apr,

day = "1",

doi = "10.1007/s11356-017-8724-z",

language = "English",

volume = "25",

pages = "10250--10263",

journal = "Environmental Science and Pollution Research",

issn = "0944-1344",

publisher = "Springer Science + Business Media",

number = "11",

}

Saratale, RG, Shin, HS, Kumar, G, Benelli, G, Ghodake, GS, Jiang, YY, Kim, DS & Saratale, GD 2018, 'Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells', Environmental Science and Pollution Research, vol. 25, no. 11, pp. 10250-10263. https://doi.org/10.1007/s11356-017-8724-z

Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells. / Saratale, Rijuta Ganesh; Shin, Han Seung; Kumar, Gopalakrishnan et al.
In: Environmental Science and Pollution Research, Vol. 25, No. 11, 01.04.2018, p. 10250-10263.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Exploiting fruit byproducts for eco-friendly nanosynthesis

T2 - Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells

AU - Saratale, Rijuta Ganesh

AU - Shin, Han Seung

AU - Kumar, Gopalakrishnan

AU - Benelli, Giovanni

AU - Ghodake, Gajanan S.

AU - Jiang, Yuan Yuan

AU - Kim, Dong Su

AU - Saratale, Ganesh Dattatraya

N1 - Funding Information: This research was supported by the Agricultural Research Center funded by the Ministry of Food, Forestry, and Fisheries, Korea. One of the author GK acknowledges Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (Grant No: 2016H1D3A1908953). The authors would like to thank Prof. Shrikrishna D. Sartale, Department of Physics, Savitribai Phule Pune University, India, for availing the HR-TEM facility. The authors are also thankful to Mr. Oh Sung-Taek, Dongguk University, for his technical help during anticancer studies. Publisher Copyright: © 2017, Springer-Verlag Berlin Heidelberg.

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Process byproducts from the fruit industry may represent a cheap and reliable source of green reducing agents to be used in current bio-nanosynthesis. This study reports the use of orange (Citrus × clementina) peel aqueous extract (OPE) for one-pot green synthesis of silver nanoparticles (AgNPs) with high effectiveness against various microbial pathogens as well as rat glial tumor C6 cells. The effects of various operational parameters on the synthesis of AgNPs were systematically investigated. The morphology, particle size, and properties of synthesized AgNPs were characterized using UV–visible spectroscopy, x-ray diffraction, x-ray photoelectron spectroscopy, field emission scanning electron microscopy, energy-dispersive x-ray spectroscopy, and Fourier transform infrared spectroscopy. High-resolution transmission electron microscopy shows that the nanoparticles are mostly spherical in shape and monodispersed, with an average particle size of 15–20 nm. Notably, the OPE-synthesized AgNPs were stable up to 6 months without change in their properties. Low doses of OPE-AgNPs inhibited the growth of human pathogens Escherichia coli, Bacillus cereus, and Staphylococcus aureus. The minimum inhibitory concentration and minimum bactericidal concentration of AgNPs against selected pathogenic bacteria were determined. OPE-AgNPs exhibited strong antioxidant activity in terms of ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging (IC50 49.6 μg/mL) and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging (IC50 63.4 μg/mL). OPE-AgNPs showed dose-dependent response against rat glial tumor C6 cells (LD50 60 μg/mL) showing a promising potential as anticancer agents. Overall, the current investigation highlighted a cheap green technology route to synthesize AgNPs using OPE byproducts and could potentially be utilized in biomedical, cosmetic, and pharmaceutical industry.

AB - Process byproducts from the fruit industry may represent a cheap and reliable source of green reducing agents to be used in current bio-nanosynthesis. This study reports the use of orange (Citrus × clementina) peel aqueous extract (OPE) for one-pot green synthesis of silver nanoparticles (AgNPs) with high effectiveness against various microbial pathogens as well as rat glial tumor C6 cells. The effects of various operational parameters on the synthesis of AgNPs were systematically investigated. The morphology, particle size, and properties of synthesized AgNPs were characterized using UV–visible spectroscopy, x-ray diffraction, x-ray photoelectron spectroscopy, field emission scanning electron microscopy, energy-dispersive x-ray spectroscopy, and Fourier transform infrared spectroscopy. High-resolution transmission electron microscopy shows that the nanoparticles are mostly spherical in shape and monodispersed, with an average particle size of 15–20 nm. Notably, the OPE-synthesized AgNPs were stable up to 6 months without change in their properties. Low doses of OPE-AgNPs inhibited the growth of human pathogens Escherichia coli, Bacillus cereus, and Staphylococcus aureus. The minimum inhibitory concentration and minimum bactericidal concentration of AgNPs against selected pathogenic bacteria were determined. OPE-AgNPs exhibited strong antioxidant activity in terms of ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging (IC50 49.6 μg/mL) and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging (IC50 63.4 μg/mL). OPE-AgNPs showed dose-dependent response against rat glial tumor C6 cells (LD50 60 μg/mL) showing a promising potential as anticancer agents. Overall, the current investigation highlighted a cheap green technology route to synthesize AgNPs using OPE byproducts and could potentially be utilized in biomedical, cosmetic, and pharmaceutical industry.

KW - ABTS and DPPH free radical scavenging activity

KW - Antimicrobial activity

KW - Green synthesis

KW - HR-TEM

KW - In vitro cytotoxicity

KW - Orange peel extract

UR - http://www.scopus.com/inward/record.url?scp=85015692057&partnerID=8YFLogxK

U2 - 10.1007/s11356-017-8724-z

DO - 10.1007/s11356-017-8724-z

M3 - Article

C2 - 28303540

AN - SCOPUS:85015692057

SN - 0944-1344

VL - 25

SP - 10250

EP - 10263

JO - Environmental Science and Pollution Research

JF - Environmental Science and Pollution Research

IS - 11

ER -

Saratale RG, Shin HS, Kumar G, Benelli G, Ghodake GS, Jiang YY et al. Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells. Environmental Science and Pollution Research. 2018 Apr 1;25(11):10250-10263. doi: 10.1007/s11356-017-8724-z