Experimental phasing using zinc anomalous scattering

Sun Shin Cha, Young Jun An, Chang Sook Jeong, Min Kyu Kim, Sung Gyu Lee, Kwang Hoon Lee, Byung Ha Oh

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.

Original languageEnglish
Pages (from-to)1253-1258
Number of pages6
JournalActa Crystallographica Section D: Biological Crystallography
Volume68
Issue number9
DOIs
StatePublished - Sep 2012

Keywords

  • phasing
  • zinc anomalous scattering
  • Zn derivatization

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