TY - JOUR
T1 - Experimental design optimization of the capillary electrophoresis separation of leucine enkephalin and its immune complex
AU - Babar, Sheikh Md Enayetul
AU - Song, Eun Joo
AU - Hasan, Md Nabiul
AU - Yoo, Young Sook
PY - 2007/9
Y1 - 2007/9
N2 - To optimize the capillary electrophoretic separation conditions for leucine enkephalin (LE) and the immune complex of the LE and anti-LE reaction, an analysis using a three-level, three-factorial Box-Behnken design was performed. Three separation parameters, buffer pH (X1), buffer concentration (X2), and applied voltage (X3), were chosen to observe the effect on separation responses. The responses were theoretical plate number, migration time of the LE peak, and resolution between the peaks. The optimum conditions and process validation were determined using statistical regression analysis and surface plot diagrams. The capillary electrophoresis optimum separation conditions were established to be 75 mM phosphate buffer at pH 7.00 with an applied separation voltage of 15 kV. By using the analysis technique, the prediction of responses was satisfactory and process verification yielded values within the ±5% range of the predicted efficiency.
AB - To optimize the capillary electrophoretic separation conditions for leucine enkephalin (LE) and the immune complex of the LE and anti-LE reaction, an analysis using a three-level, three-factorial Box-Behnken design was performed. Three separation parameters, buffer pH (X1), buffer concentration (X2), and applied voltage (X3), were chosen to observe the effect on separation responses. The responses were theoretical plate number, migration time of the LE peak, and resolution between the peaks. The optimum conditions and process validation were determined using statistical regression analysis and surface plot diagrams. The capillary electrophoresis optimum separation conditions were established to be 75 mM phosphate buffer at pH 7.00 with an applied separation voltage of 15 kV. By using the analysis technique, the prediction of responses was satisfactory and process verification yielded values within the ±5% range of the predicted efficiency.
KW - Box - Behnken design
KW - Capillary electrophoresis
KW - Leucine enkephalin
UR - http://www.scopus.com/inward/record.url?scp=34848845219&partnerID=8YFLogxK
U2 - 10.1002/jssc.200700145
DO - 10.1002/jssc.200700145
M3 - Article
C2 - 17688300
AN - SCOPUS:34848845219
SN - 1615-9306
VL - 30
SP - 2311
EP - 2319
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 14
ER -