Abstract
We present a microfluidics device for the capture and release of circulating exosome from blood. The EXODIF, composed of two distinct immuno-patterned layers, is capable to enhance binding chance between antibody and exosome and generates mechanical whirling; thus achieving high-throughput and specific exosome isolation. The device performance was verified using blood from cancer patients and healthy donors, revealed that cancer patient show significantly higher exosome level compared to healthy donors after electron microscopy and nanoparticle tracking analysis. This simple and fast exosome isolation is appropriate for exosome-based cancer diagnostics and may help to elucidate the function of exosome in cancer.
Original language | English |
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Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
Publisher | Chemical and Biological Microsystems Society |
Pages | 317-318 |
Number of pages | 2 |
ISBN (Electronic) | 9780979806490 |
State | Published - 2016 |
Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 |
Publication series
Name | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
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Conference
Conference | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
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Country/Territory | Ireland |
City | Dublin |
Period | 9/10/16 → 13/10/16 |
Bibliographical note
Funding Information:This research was supported by the Converging Research Center Program funded by the Ministry of Science, ICT and Future Planning (Project No. 2015054201)
Keywords
- Cancer
- Dual immuno-patterns
- Exosome
- Extracellular vesicles
- Isolations