Exosome-specific dual-patterned immuno-filtration (EXODIF) devices for the rapid capture and efficient release of circulating exosome from cancer patients' blood

Y. T. Kang; Y. J. Kim; J. Bu; I. Doh; Y. H. Cho; S. W. Han; B. I. Moon

Exosome-specific dual-patterned immuno-filtration (EXODIF) devices for the rapid capture and efficient release of circulating exosome from cancer patients' blood

Y. T. Kang, Y. J. Kim, J. Bu, I. Doh, Y. H. Cho, S. W. Han, B. I. Moon

Department of Surgery

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We present a microfluidics device for the capture and release of circulating exosome from blood. The EXODIF, composed of two distinct immuno-patterned layers, is capable to enhance binding chance between antibody and exosome and generates mechanical whirling; thus achieving high-throughput and specific exosome isolation. The device performance was verified using blood from cancer patients and healthy donors, revealed that cancer patient show significantly higher exosome level compared to healthy donors after electron microscopy and nanoparticle tracking analysis. This simple and fast exosome isolation is appropriate for exosome-based cancer diagnostics and may help to elucidate the function of exosome in cancer.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	317-318
Number of pages	2
ISBN (Electronic)	9780979806490
State	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016

Publication series

Name	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	9/10/16 → 13/10/16

Bibliographical note

Funding Information:
This research was supported by the Converging Research Center Program funded by the Ministry of Science, ICT and Future Planning (Project No. 2015054201)

Keywords

Cancer
Dual immuno-patterns
Exosome
Extracellular vesicles
Isolations

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Kang, Y. T., Kim, Y. J., Bu, J., Doh, I., Cho, Y. H., Han, S. W., & Moon, B. I. (2016). Exosome-specific dual-patterned immuno-filtration (EXODIF) devices for the rapid capture and efficient release of circulating exosome from cancer patients' blood. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 317-318). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.

Kang, Y. T. ; Kim, Y. J. ; Bu, J. et al. / Exosome-specific dual-patterned immuno-filtration (EXODIF) devices for the rapid capture and efficient release of circulating exosome from cancer patients' blood. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 317-318 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

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title = "Exosome-specific dual-patterned immuno-filtration (EXODIF) devices for the rapid capture and efficient release of circulating exosome from cancer patients' blood",

abstract = "We present a microfluidics device for the capture and release of circulating exosome from blood. The EXODIF, composed of two distinct immuno-patterned layers, is capable to enhance binding chance between antibody and exosome and generates mechanical whirling; thus achieving high-throughput and specific exosome isolation. The device performance was verified using blood from cancer patients and healthy donors, revealed that cancer patient show significantly higher exosome level compared to healthy donors after electron microscopy and nanoparticle tracking analysis. This simple and fast exosome isolation is appropriate for exosome-based cancer diagnostics and may help to elucidate the function of exosome in cancer.",

keywords = "Cancer, Dual immuno-patterns, Exosome, Extracellular vesicles, Isolations",

author = "Kang, {Y. T.} and Kim, {Y. J.} and J. Bu and I. Doh and Cho, {Y. H.} and Han, {S. W.} and Moon, {B. I.}",

note = "Funding Information: This research was supported by the Converging Research Center Program funded by the Ministry of Science, ICT and Future Planning (Project No. 2015054201); 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

year = "2016",

language = "English",

series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

publisher = "Chemical and Biological Microsystems Society",

pages = "317--318",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

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Kang, YT, Kim, YJ, Bu, J, Doh, I, Cho, YH, Han, SW & Moon, BI 2016, Exosome-specific dual-patterned immuno-filtration (EXODIF) devices for the rapid capture and efficient release of circulating exosome from cancer patients' blood. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 317-318, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 9/10/16.

Exosome-specific dual-patterned immuno-filtration (EXODIF) devices for the rapid capture and efficient release of circulating exosome from cancer patients' blood. / Kang, Y. T.; Kim, Y. J.; Bu, J. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 317-318 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Exosome-specific dual-patterned immuno-filtration (EXODIF) devices for the rapid capture and efficient release of circulating exosome from cancer patients' blood

AU - Kang, Y. T.

AU - Kim, Y. J.

AU - Bu, J.

AU - Doh, I.

AU - Cho, Y. H.

AU - Han, S. W.

AU - Moon, B. I.

N1 - Funding Information: This research was supported by the Converging Research Center Program funded by the Ministry of Science, ICT and Future Planning (Project No. 2015054201)

PY - 2016

Y1 - 2016

N2 - We present a microfluidics device for the capture and release of circulating exosome from blood. The EXODIF, composed of two distinct immuno-patterned layers, is capable to enhance binding chance between antibody and exosome and generates mechanical whirling; thus achieving high-throughput and specific exosome isolation. The device performance was verified using blood from cancer patients and healthy donors, revealed that cancer patient show significantly higher exosome level compared to healthy donors after electron microscopy and nanoparticle tracking analysis. This simple and fast exosome isolation is appropriate for exosome-based cancer diagnostics and may help to elucidate the function of exosome in cancer.

AB - We present a microfluidics device for the capture and release of circulating exosome from blood. The EXODIF, composed of two distinct immuno-patterned layers, is capable to enhance binding chance between antibody and exosome and generates mechanical whirling; thus achieving high-throughput and specific exosome isolation. The device performance was verified using blood from cancer patients and healthy donors, revealed that cancer patient show significantly higher exosome level compared to healthy donors after electron microscopy and nanoparticle tracking analysis. This simple and fast exosome isolation is appropriate for exosome-based cancer diagnostics and may help to elucidate the function of exosome in cancer.

KW - Cancer

KW - Dual immuno-patterns

KW - Exosome

KW - Extracellular vesicles

KW - Isolations

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M3 - Conference contribution

AN - SCOPUS:85014205368

T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

SP - 317

EP - 318

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Y2 - 9 October 2016 through 13 October 2016

ER -

Kang YT, Kim YJ, Bu J, Doh I, Cho YH, Han SW et al. Exosome-specific dual-patterned immuno-filtration (EXODIF) devices for the rapid capture and efficient release of circulating exosome from cancer patients' blood. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 317-318. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Exosome-specific dual-patterned immuno-filtration (EXODIF) devices for the rapid capture and efficient release of circulating exosome from cancer patients' blood

Abstract

Publication series

Conference

Bibliographical note

Keywords

UN SDGs

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Cite this