Exosome detection via the ultrafast-isolation system: EXODUS

Yuchao Chen, Qingfu Zhu, Liming Cheng, Yong Wang, Meng Li, Qinsi Yang, Liang Hu, Doudou Lou, Jiaoyuan Li, Xianjun Dong, Luke P. Lee, Fei Liu

Research output: Contribution to journalArticlepeer-review

210 Scopus citations

Abstract

Exosomes have shown great potential in disease diagnostics and therapeutics. However, current isolation approaches are burdensome and suffer from low speed, yield and purity, limiting basic research and clinical applications. Here, we describe an efficient exosome detection method via the ultrafast-isolation system (EXODUS) that allows automated label-free purification of exosomes from varied biofluids. We obtained the ultra-efficient purification of exosomes by negative pressure oscillation and double coupled harmonic oscillator–enabled membrane vibration. Our two coupled oscillators generate dual-frequency transverse waves on the membranes, enabling EXODUS to outperform other isolation techniques in speed, purity and yield. We demonstrated EXODUS by purifying exosomes from urine samples of 113 patients and validated the practical relevance in exosomal RNA profiling with the high-resolution capability and high-throughput analysis.

Original languageEnglish
Pages (from-to)212-218
Number of pages7
JournalNature Methods
Volume18
Issue number2
DOIs
StatePublished - Feb 2021

Bibliographical note

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature America, Inc.

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