Excimers beyond pyrene: A far-red optical proximity reporter and its application to the label-free detection of DNA

Garam Han, Dongwook Kim, Younbong Park, Jean Bouffard, Youngmi Kim

Research output: Contribution to journalArticlepeer-review

106 Scopus citations

Abstract

A family of organic chromophores that, like pyrene, forms emissive excimers is reported. Their chemical and photophysical properties are superior to pyrene for the design of chemo- and biosensors. Unlike hydrophobic pyrene, which requires excitation by cell-damaging UV irradiation, these polar dyes absorb strongly in the visible range, and their excimers emit brightly in the red to far-red region of the electromagnetic spectrum. The intensity of the emission signal is greatly increased upon formation of a preassociated dimer that is triggered upon aggregation or crystallization. In demonstration of the potential of this new family of excimer-forming dyes, a probe that is capable of detecting label-free DNA in water down to 10 pM and also doubles as a visualization agent for DNA in gel electrophoresis is reported.

Original languageEnglish
Pages (from-to)3912-3916
Number of pages5
JournalAngewandte Chemie - International Edition
Volume54
Issue number13
DOIs
StatePublished - 23 Mar 2015

Keywords

  • aggregation
  • chromophores
  • DNA detection
  • excimers
  • fluorescence

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