Abstract
We present a microfluidic device for performing impedance spectroscopic analyses of the steady-state and time-transient response of single cells exposed to cytotoxic agents and drug compounds. This platform is capable of long-term label-free assays. Sample preparation steps normally associated with fluorescence analysis (fixing, staining and washing) are avoided. We analysed the transient impedance from single cells to study the kinetics of cell response to Streptolysin-O (SLO) on mammalian cells. The toxin was found to cause a gradual decrease in the impedance, which corresponded to an increase in membrane conductivity. Increasing concentrations of toxins caused a higher rate of change seen as an overall decrease in the impedance.
| Original language | English |
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| Title of host publication | Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
| Editors | Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1083-1085 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806407 |
| State | Published - 2007 |
| Event | 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France Duration: 7 Oct 2007 → 11 Oct 2007 |
Publication series
| Name | Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
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Conference
| Conference | 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
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| Country/Territory | France |
| City | Paris |
| Period | 7/10/07 → 11/10/07 |
Bibliographical note
Publisher Copyright:© 2007 CBMS.
Keywords
- Impedance Spectroscopy
- Label Free Method
- Single Cell Analysis