Evidence for Heme Oxygenase-1 Association with Caveolin-1 and -2 in Mouse Mesangial Cells

Nam Hee Jung, Hong Pyo Kim, Bum Rae Kim, Seok Ho Cha, Gyeong A. Kim, Hunjoo Ha, Young Eun Na, Young Nam Cha

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40 Scopus citations

Abstract

The interaction of heme oxygenase-1 (HO-1) and caveolin in the cultured mouse mesangial cells (MMC) was investigated. In normal MMCs, high levels of caveolin-2 and low level of caveolin-1 at mRNA and protein level were observed without any detectable expression of caveolin-3. Upon treating the MMCs either with cadmium (Cd) or spermine NONOate (SPER/NO), expression of HO-1 mRNA and protein was increased. Caveolae rich membranous fractions from the MMCs treated with Cd or SPER/NO contained both HO-1 and caveolin-1 or caveolin-2. The experiments of immuno-precipitation showed complex formation between the HO-1 and caveolin-1 or caveolin-2 in the Cd treated MMCs. Confocal microscopic results also support co-localization of HO-1 and caveolin-1 or caveolin-2 at the plasma membrane. Co-localization of caveolins with HO-1 in caveolae suggested that caveolin could also play an important role in regulating the function of HO-1.

Original languageEnglish
Pages (from-to)525-532
Number of pages8
JournalIUBMB Life
Volume55
Issue number9
DOIs
StatePublished - Sep 2003

Keywords

  • Cadmium
  • Caveolin
  • Heme oxygenase-1
  • Mouse mesangial cell
  • Nitric Oxide

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