Evaluation of inhibition of miRNA expression induced by anti-miRNA oligonucleotides

Dong Kyu Chae; Eunmi Ban; Young Sook Yoo; Ja‐Hyun ‐H Baik; Eun Joo Song

doi:10.1007/s00216-016-9611-z

Evaluation of inhibition of miRNA expression induced by anti-miRNA oligonucleotides

Dong Kyu Chae, Eunmi Ban, Young Sook Yoo, Ja‐Hyun ‐H Baik, Eun Joo Song

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

MicroRNAs (miRNAs) are short RNA molecules that control the expression of mRNAs associated with various biological processes. Therefore, deregulated miRNAs play an important role in the pathogenesis of diseases. Numerous studies aimed at developing novel miRNA-based drugs or determining miRNA functions have been conducted by inhibiting miRNAs using anti-miRNA oligonucleotides (AMOs), which inhibit the function by hybridizing with miRNA. To increase the binding affinity and specificity to target miRNA, AMOs with various chemical modifications have been developed. Evaluating the potency of these various types of AMOs is an essential step in their development. In this study, we developed a capillary electrophoresis with laser-induced fluorescence (CE-LIF) method to evaluate the potency of AMOs by measuring changes in miRNA levels with fluorescence-labeled ssDNA probes using AMO-miR-23a, which inhibits miR-23a related to lung cancer. In order to eliminate interference by excess AMOs during hybridization of the ssDNA probe with the miR-23a, the concentration of the ssDNA probe was optimized. This newly developed method was used to compare the potency of two different modified AMOs. The data were supported by the results of a luciferase assay. This study demonstrated that CE-LIF analysis could be used to accurately evaluate AMO potency in biological samples.

Original language	English
Pages (from-to)	4829-4833
Number of pages	5
Journal	Analytical and Bioanalytical Chemistry
Volume	408
Issue number	18
DOIs	https://doi.org/10.1007/s00216-016-9611-z
State	Published - 1 Jul 2016

Bibliographical note

Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.

Keywords

Anti-miRNA oligonucleotide
Capillary electrophoresis with laser-induced fluorescence
miR-23a
miRNA

Access to Document

10.1007/s00216-016-9611-z

Cite this

@article{790f2f4167f246d2aff1e8160d105399,

title = "Evaluation of inhibition of miRNA expression induced by anti-miRNA oligonucleotides",

abstract = "MicroRNAs (miRNAs) are short RNA molecules that control the expression of mRNAs associated with various biological processes. Therefore, deregulated miRNAs play an important role in the pathogenesis of diseases. Numerous studies aimed at developing novel miRNA-based drugs or determining miRNA functions have been conducted by inhibiting miRNAs using anti-miRNA oligonucleotides (AMOs), which inhibit the function by hybridizing with miRNA. To increase the binding affinity and specificity to target miRNA, AMOs with various chemical modifications have been developed. Evaluating the potency of these various types of AMOs is an essential step in their development. In this study, we developed a capillary electrophoresis with laser-induced fluorescence (CE-LIF) method to evaluate the potency of AMOs by measuring changes in miRNA levels with fluorescence-labeled ssDNA probes using AMO-miR-23a, which inhibits miR-23a related to lung cancer. In order to eliminate interference by excess AMOs during hybridization of the ssDNA probe with the miR-23a, the concentration of the ssDNA probe was optimized. This newly developed method was used to compare the potency of two different modified AMOs. The data were supported by the results of a luciferase assay. This study demonstrated that CE-LIF analysis could be used to accurately evaluate AMO potency in biological samples.",

keywords = "Anti-miRNA oligonucleotide, Capillary electrophoresis with laser-induced fluorescence, miR-23a, miRNA",

author = "Chae, {Dong Kyu} and Eunmi Ban and Yoo, {Young Sook} and Baik, {Ja‐Hyun ‐H} and Song, {Eun Joo}",

note = "Publisher Copyright: {\textcopyright} 2016, Springer-Verlag Berlin Heidelberg.",

year = "2016",

month = jul,

day = "1",

doi = "10.1007/s00216-016-9611-z",

language = "English",

volume = "408",

pages = "4829--4833",

journal = "Analytical and Bioanalytical Chemistry",

issn = "1618-2642",

publisher = "Springer Verlag",

number = "18",

}

TY - JOUR

T1 - Evaluation of inhibition of miRNA expression induced by anti-miRNA oligonucleotides

AU - Chae, Dong Kyu

AU - Ban, Eunmi

AU - Yoo, Young Sook

AU - Baik, Ja‐Hyun ‐H

AU - Song, Eun Joo

PY - 2016/7/1

Y1 - 2016/7/1

N2 - MicroRNAs (miRNAs) are short RNA molecules that control the expression of mRNAs associated with various biological processes. Therefore, deregulated miRNAs play an important role in the pathogenesis of diseases. Numerous studies aimed at developing novel miRNA-based drugs or determining miRNA functions have been conducted by inhibiting miRNAs using anti-miRNA oligonucleotides (AMOs), which inhibit the function by hybridizing with miRNA. To increase the binding affinity and specificity to target miRNA, AMOs with various chemical modifications have been developed. Evaluating the potency of these various types of AMOs is an essential step in their development. In this study, we developed a capillary electrophoresis with laser-induced fluorescence (CE-LIF) method to evaluate the potency of AMOs by measuring changes in miRNA levels with fluorescence-labeled ssDNA probes using AMO-miR-23a, which inhibits miR-23a related to lung cancer. In order to eliminate interference by excess AMOs during hybridization of the ssDNA probe with the miR-23a, the concentration of the ssDNA probe was optimized. This newly developed method was used to compare the potency of two different modified AMOs. The data were supported by the results of a luciferase assay. This study demonstrated that CE-LIF analysis could be used to accurately evaluate AMO potency in biological samples.

AB - MicroRNAs (miRNAs) are short RNA molecules that control the expression of mRNAs associated with various biological processes. Therefore, deregulated miRNAs play an important role in the pathogenesis of diseases. Numerous studies aimed at developing novel miRNA-based drugs or determining miRNA functions have been conducted by inhibiting miRNAs using anti-miRNA oligonucleotides (AMOs), which inhibit the function by hybridizing with miRNA. To increase the binding affinity and specificity to target miRNA, AMOs with various chemical modifications have been developed. Evaluating the potency of these various types of AMOs is an essential step in their development. In this study, we developed a capillary electrophoresis with laser-induced fluorescence (CE-LIF) method to evaluate the potency of AMOs by measuring changes in miRNA levels with fluorescence-labeled ssDNA probes using AMO-miR-23a, which inhibits miR-23a related to lung cancer. In order to eliminate interference by excess AMOs during hybridization of the ssDNA probe with the miR-23a, the concentration of the ssDNA probe was optimized. This newly developed method was used to compare the potency of two different modified AMOs. The data were supported by the results of a luciferase assay. This study demonstrated that CE-LIF analysis could be used to accurately evaluate AMO potency in biological samples.

KW - Anti-miRNA oligonucleotide

KW - Capillary electrophoresis with laser-induced fluorescence

KW - miR-23a

KW - miRNA

UR - http://www.scopus.com/inward/record.url?scp=84968639128&partnerID=8YFLogxK

U2 - 10.1007/s00216-016-9611-z

DO - 10.1007/s00216-016-9611-z

M3 - Article

C2 - 27178549

AN - SCOPUS:84968639128

SN - 1618-2642

VL - 408

SP - 4829

EP - 4833

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 18

ER -

Evaluation of inhibition of miRNA expression induced by anti-miRNA oligonucleotides

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this