TY - JOUR
T1 - Estimation of multiexponential fluorescence decay parameters using compressive sensing
AU - Yang, Sejung
AU - Lee, Joohyun
AU - Lee, Youmin
AU - Lee, Minyung
AU - Lee, Byung Uk
N1 - Publisher Copyright:
© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Fluorescence lifetime imaging microscopy (FLIM) is a microscopic imaging technique to present an image of fluorophore lifetimes. It circumvents the problems of typical imaging methods such as intensity attenuation from depth since a lifetime is independent of the excitation intensity or fluorophore concentration. The lifetime is estimated from the time sequence of photon counts observed with signal-dependent noise, which has a Poisson distribution. Conventional methods usually estimate single or biexponential decay parameters. However, a lifetime component has a distribution or width, because the lifetime depends on macromolecular conformation or inhomogeneity. We present a novel algorithm based on a sparse representation which can estimate the distribution of lifetime. We verify the enhanced performance through simulations and experiments.
AB - Fluorescence lifetime imaging microscopy (FLIM) is a microscopic imaging technique to present an image of fluorophore lifetimes. It circumvents the problems of typical imaging methods such as intensity attenuation from depth since a lifetime is independent of the excitation intensity or fluorophore concentration. The lifetime is estimated from the time sequence of photon counts observed with signal-dependent noise, which has a Poisson distribution. Conventional methods usually estimate single or biexponential decay parameters. However, a lifetime component has a distribution or width, because the lifetime depends on macromolecular conformation or inhomogeneity. We present a novel algorithm based on a sparse representation which can estimate the distribution of lifetime. We verify the enhanced performance through simulations and experiments.
KW - compressive sensing
KW - fluorescence decay parameters
KW - fluorescence lifetime imaging microscopy
KW - multiexponential decay
UR - http://www.scopus.com/inward/record.url?scp=84942694194&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.20.9.096003
DO - 10.1117/1.JBO.20.9.096003
M3 - Article
C2 - 26334976
AN - SCOPUS:84942694194
SN - 1083-3668
VL - 20
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 9
M1 - 096003
ER -