Estimation of multiexponential fluorescence decay parameters using compressive sensing

Sejung Yang, Joohyun Lee, Youmin Lee, Minyung Lee, Byung Uk Lee

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Fluorescence lifetime imaging microscopy (FLIM) is a microscopic imaging technique to present an image of fluorophore lifetimes. It circumvents the problems of typical imaging methods such as intensity attenuation from depth since a lifetime is independent of the excitation intensity or fluorophore concentration. The lifetime is estimated from the time sequence of photon counts observed with signal-dependent noise, which has a Poisson distribution. Conventional methods usually estimate single or biexponential decay parameters. However, a lifetime component has a distribution or width, because the lifetime depends on macromolecular conformation or inhomogeneity. We present a novel algorithm based on a sparse representation which can estimate the distribution of lifetime. We verify the enhanced performance through simulations and experiments.

Original languageEnglish
Article number096003
JournalJournal of Biomedical Optics
Issue number9
StatePublished - 1 Sep 2015

Bibliographical note

Publisher Copyright:
© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.


  • compressive sensing
  • fluorescence decay parameters
  • fluorescence lifetime imaging microscopy
  • multiexponential decay


Dive into the research topics of 'Estimation of multiexponential fluorescence decay parameters using compressive sensing'. Together they form a unique fingerprint.

Cite this