Estimation of multiexponential fluorescence decay parameters using compressive sensing

Sejung Yang, Joohyun Lee, Youmin Lee, Minyung Lee, Byung Uk Lee

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a microscopic imaging technique to present an image of fluorophore lifetimes. It circumvents the problems of typical imaging methods such as intensity attenuation from depth since a lifetime is independent of the excitation intensity or fluorophore concentration. The lifetime is estimated from the time sequence of photon counts observed with signal-dependent noise, which has a Poisson distribution. Conventional methods usually estimate single or biexponential decay parameters. However, a lifetime component has a distribution or width, because the lifetime depends on macromolecular conformation or inhomogeneity. We present a novel algorithm based on a sparse representation which can estimate the distribution of lifetime. We verify the enhanced performance through simulations and experiments.

Original languageEnglish
Article number096003
JournalJournal of Biomedical Optics
Volume20
Issue number9
DOIs
StatePublished - 1 Sep 2015

Keywords

  • compressive sensing
  • fluorescence decay parameters
  • fluorescence lifetime imaging microscopy
  • multiexponential decay

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