Epidermal Growth Factor Receptor-Targeted Multifunctional Photosensitizers for Bladder Cancer Imaging and Photodynamic Therapy

Ravindra R. Cheruku, Joseph Cacaccio, Farukh A. Durrani, Walter A. Tabaczynski, Ramona Watson, Aimee Marko, Rahul Kumar, Mohamed E. El-Khouly, Shunichi Fukuzumi, Joseph R. Missert, Rutao Yao, Munawwar Sajjad, Dhyan Chandra, Khurshid Guru, Ravindra K. Pandey

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The in vitro and in vivo anticancer activity of iodinated photosensitizers (PSs) with and without an erlotinib moiety was investigated in UMUC3 [epidermal growth factor (EGFR)-positive] and T24 (EGFR-low) cell lines and tumored mice. Both the erlotinib-conjugated PSs 3 and 5 showed EGFR target specificity, but the position-3 erlotinib-PS conjugate 3 demonstrated lower photodynamic therapy efficacy than the corresponding non-erlotinib analogue 1, whereas the conjugate 5 containing an erlotinib moiety at position-17 of the PS showed higher tumor uptake and long-term tumor cure (severe combined immunodeficient mice bearing UMUC3 tumors). PS-erlotinib conjugates in the absence of light were ineffective in vitro and in vivo, but robust apoptotic and necrotic cell death was observed in bladder cancer cells after exposing them to a laser light at 665 nm. In contrast to 18F-fluorodeoxyglucose, a positron emission tomography agent, the position-17 erlotinib conjugate (124I-analogue 6) showed enhanced UMUC3 tumor contrast even at a low imaging dose of 15 μCi/mouse.

Original languageEnglish
Pages (from-to)2598-2617
Number of pages20
JournalJournal of Medicinal Chemistry
Volume62
Issue number5
DOIs
StatePublished - 14 Mar 2019

Bibliographical note

Publisher Copyright:
© 2019 American Chemical Society.

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