Enzyme/whole-cell biotransformation of plant oils, yeast derived oils, and microalgae fatty acid methyl esters into n-nonanoic acid, 9-hydroxynonanoic acid, and 1,9-nonanedioic acid

Eun Ji Seo, Young Joo Yeon, Joo Hyun Seo, Jung Hoo Lee, Jhoanne P. Boñgol, Yuri Oh, Jong Moon Park, Sang Min Lim, Choul Gyun Lee, Jin Byung Park

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Oils and fatty acids are important renewable resources provided by nature. Therefore, biotransformation of renewable oils and fatty acids into industrially relevant C9 chemicals was investigated in this study. Olive oil, soybean oil, yeast derived oil, and microalgae fatty acid methyl esters were converted into n-nonanoic acid, 9-hydroxynonanoic acid, and 1,9-nonanedioic acid by a lipase and a recombinant Escherichia coli expressing oleate hydratase, long chain secondary alcohol dehydrogenase, Baeyer-Villiger monooxygenase, long chain primary alcohol dehydrogenase, and aldehyde dehydrogenase. It was found that n-nonanoic acid and azelaic acid could be produced to a concentration of 4.3 mM from 3 g/L olive oil with a specific product formation rate of 3.1 U/g dry cells. Biotransformation rates were influenced by compositions of fatty acids and purity of the starting material. This study may contribute to the production of industrially relevant C9 chemicals from renewable oils and fatty acids by simultaneous enzyme/whole-cell biotransformation.

Original languageEnglish
Pages (from-to)288-294
Number of pages7
JournalBioresource Technology
Volume251
DOIs
StatePublished - Mar 2018

Keywords

  • Biotransformation
  • C9 chemicals
  • Escherichia coli
  • Microalgae fatty acid methyl esters
  • Plant oils
  • Yeast derived oils

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