Oils and fatty acids are important renewable resources provided by nature. Therefore, biotransformation of renewable oils and fatty acids into industrially relevant C9 chemicals was investigated in this study. Olive oil, soybean oil, yeast derived oil, and microalgae fatty acid methyl esters were converted into n-nonanoic acid, 9-hydroxynonanoic acid, and 1,9-nonanedioic acid by a lipase and a recombinant Escherichia coli expressing oleate hydratase, long chain secondary alcohol dehydrogenase, Baeyer-Villiger monooxygenase, long chain primary alcohol dehydrogenase, and aldehyde dehydrogenase. It was found that n-nonanoic acid and azelaic acid could be produced to a concentration of 4.3 mM from 3 g/L olive oil with a specific product formation rate of 3.1 U/g dry cells. Biotransformation rates were influenced by compositions of fatty acids and purity of the starting material. This study may contribute to the production of industrially relevant C9 chemicals from renewable oils and fatty acids by simultaneous enzyme/whole-cell biotransformation.
Bibliographical noteFunding Information:
This study was supported by a Marine Biomaterials Research Center grant (No. D11013214H480000100 ) from the Marine Biotechnology Program funded by the Ministry of Oceans and Fisheries, Republic of Korea. It was also supported by a grant (No. 2017008670 ) of the National Research Foundation of Korea (NRF) funded by the Korea government (MEST).
© 2017 Elsevier Ltd
- C9 chemicals
- Escherichia coli
- Microalgae fatty acid methyl esters
- Plant oils
- Yeast derived oils