Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles

Hong Jai Lee, Hee Ho Park, Jeong Ah Kim, Ju Hyun Park, Jina Ryu, Jeongseon Choi, Jongmin Lee, Won Jong Rhee, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Nanoparticles have been widely used for delivering various chemical and biomolecular drugs, such as anti-cancer drugs and therapeutic proteins. Among nanoparticles, protein nanoparticles have advantages of non-cytotoxicity and biodegradability. In this study, a recombinant 30Kc19 protein was applied to human serum albumin (HSA) nanoparticles to enhance cellular uptake and stability of a nanoparticle cargo enzyme. The 30Kc19 protein, which originates from silkworm, has cell-penetrating and enzyme-stabilizing abilities. Therefore, 30Kc19-HSA nanoparticles were expected to enhance cellular uptake and stability of an enzyme loaded on the nanoparticles. Here, nanoparticles loaded with β-galactosidase were prepared using the desolvation method. The 30Kc19-HSA nanoparticles were uniformly spherical in shape, dispersed evenly in phosphate buffered saline and cell culture media, and released β-galactosidase in a sustained manner. The 30Kc19-HSA nanoparticles had negligible toxicity to animal cells and exhibited enhanced cellular uptake and intracellular stability of β-galactosidase in HeLa and HEK293 cells when compared with those of HSA nanoparticles. These results suggest that 30Kc19-HSA protein nanoparticles could be used as a versatile tool for drug delivery to various cells.

Original languageEnglish
Pages (from-to)1696-1704
Number of pages9
JournalBiomaterials
Volume35
Issue number5
DOIs
StatePublished - Feb 2014

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, & Future Planning ( 2013069511 , 2013005843 ).

Keywords

  • Drug delivery
  • Drug release
  • Enzyme stability
  • Protein
  • Protein nanoparticle

Fingerprint

Dive into the research topics of 'Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles'. Together they form a unique fingerprint.

Cite this