Enzyme delivery using protein-stabilizing and cell-penetrating 30Kc19α protein nanoparticles

Hee Ho Park, Yeon Hwa Woo, Jina Ryu, Hong Jai Lee, Ju Hyun Park, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Nanoparticles (NPs) are an emerging strategy for drug delivery and have been studied for the delivery of various biomolecules, such as chemically synthesized drugs and therapeutic proteins. In particular, protein NPs are non-cytotoxic and biodegradable. Application of a full length recombinant 30Kc19 protein to human serum albumin (HSA) NPs has been shown to improve the cellular uptake and stability of the cargo enzyme. In this study, we demonstrate that drug delivery can be achieved with only the α-helix domain of the 30Kc19 protein (30Kc19α), and without the addition of HSA. Protein concentration and pH were crucial for NP generation. NPs had a uniformly spherical shape with an optimal diameter of 180–230 nm, and released β-galactosidase in a sustained manner. The 30Kc19α protein provided stability to the cargo enzyme, and helped maintain the specific activity of the enzyme. X-gal staining showed effective delivery of β-galactosidase into human dermal fibroblasts. Non-cytotoxic property of the 30Kc19α protein demonstrates that such NPs could be a resourceful tool for delivering drugs to cells.

Original languageEnglish
Pages (from-to)76-83
Number of pages8
JournalProcess Biochemistry
StatePublished - Dec 2017

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, & Future Planning ( 2012M3A9C6050100 , 2015R1C1A1A01052831 ). This study was also supported by 2016 Research Grant from Kangwon National University .

Publisher Copyright:
© 2017 Elsevier Ltd


  • 30Kc19α
  • Cell-penetrating property
  • Drug delivery
  • Enzyme-stabilizing property
  • Protein nanoparticle
  • Soluble expression


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