Enzymatically degradable, starch-based layer-by-layer films: Application to cytocompatible single-cell nanoencapsulation

Hee Chul Moon, Sol Han, João Borges, Tamagno Pesqueira, Hyunwoo Choi, Sang Yeong Han, Hyeoncheol Cho, Ji Hun Park, João F. Mano, Insung S. Choi

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.

Original languageEnglish
Pages (from-to)6063-6071
Number of pages9
JournalSoft Matter
Issue number26
StatePublished - 14 Jul 2020

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2018R1C1B5045778), Programa Operacional Regional do Centro – Centro 2020, in the component FEDER, and by national funds (OE) through FCT/MCTES, in the scope of the project ‘‘SUPRASORT’’ (PTDC/QUI-OUT/30658/2017, CENTRO-01-0145-FEDER-030658), as well as by the European Research Council grant agreement ERC-2014-ADG-669858 (ATLAS). J. Borges gratefully acknowledges FCT for the individual contract (CEECIND/ 03202/2017). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Foundation for Science and Technology/MCTES.

Publisher Copyright:
© The Royal Society of Chemistry.


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