Enzymatically degradable, starch-based layer-by-layer films: Application to cytocompatible single-cell nanoencapsulation

Hee Chul Moon; Sol Han; João Borges; Tamagno Pesqueira; Hyunwoo Choi; Sang Yeong Han; Hyeoncheol Cho; Ji Hun Park; João F. Mano; Insung S. Choi

doi:10.1039/d0sm00876a

Enzymatically degradable, starch-based layer-by-layer films: Application to cytocompatible single-cell nanoencapsulation

Hee Chul Moon, Sol Han, João Borges, Tamagno Pesqueira, Hyunwoo Choi, Sang Yeong Han, Hyeoncheol Cho, Ji Hun Park, João F. Mano, Insung S. Choi

Department of Science Education

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.

Original language	English
Pages (from-to)	6063-6071
Number of pages	9
Journal	Soft Matter
Volume	16
Issue number	26
DOIs	https://doi.org/10.1039/d0sm00876a
State	Published - 14 Jul 2020

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2018R1C1B5045778), Programa Operacional Regional do Centro – Centro 2020, in the component FEDER, and by national funds (OE) through FCT/MCTES, in the scope of the project ‘‘SUPRASORT’’ (PTDC/QUI-OUT/30658/2017, CENTRO-01-0145-FEDER-030658), as well as by the European Research Council grant agreement ERC-2014-ADG-669858 (ATLAS). J. Borges gratefully acknowledges FCT for the individual contract (CEECIND/ 03202/2017). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Foundation for Science and Technology/MCTES.

Publisher Copyright:
© The Royal Society of Chemistry.

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@article{65802cdab2fc4d8c9c4b683a5a3d0e7d,

title = "Enzymatically degradable, starch-based layer-by-layer films: Application to cytocompatible single-cell nanoencapsulation",

abstract = "The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.",

author = "Moon, {Hee Chul} and Sol Han and Jo{\~a}o Borges and Tamagno Pesqueira and Hyunwoo Choi and Han, {Sang Yeong} and Hyeoncheol Cho and Park, {Ji Hun} and Mano, {Jo{\~a}o F.} and Choi, {Insung S.}",

note = "Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2018R1C1B5045778), Programa Operacional Regional do Centro – Centro 2020, in the component FEDER, and by national funds (OE) through FCT/MCTES, in the scope of the project {\textquoteleft}{\textquoteleft}SUPRASORT{\textquoteright}{\textquoteright} (PTDC/QUI-OUT/30658/2017, CENTRO-01-0145-FEDER-030658), as well as by the European Research Council grant agreement ERC-2014-ADG-669858 (ATLAS). J. Borges gratefully acknowledges FCT for the individual contract (CEECIND/ 03202/2017). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Foundation for Science and Technology/MCTES. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

year = "2020",

month = jul,

day = "14",

doi = "10.1039/d0sm00876a",

language = "English",

volume = "16",

pages = "6063--6071",

journal = "Soft Matter",

issn = "1744-683X",

publisher = "Royal Society of Chemistry",

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T1 - Enzymatically degradable, starch-based layer-by-layer films

T2 - Application to cytocompatible single-cell nanoencapsulation

AU - Moon, Hee Chul

AU - Han, Sol

AU - Borges, João

AU - Pesqueira, Tamagno

AU - Choi, Hyunwoo

AU - Han, Sang Yeong

AU - Cho, Hyeoncheol

AU - Park, Ji Hun

AU - Mano, João F.

AU - Choi, Insung S.

N1 - Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2018R1C1B5045778), Programa Operacional Regional do Centro – Centro 2020, in the component FEDER, and by national funds (OE) through FCT/MCTES, in the scope of the project ‘‘SUPRASORT’’ (PTDC/QUI-OUT/30658/2017, CENTRO-01-0145-FEDER-030658), as well as by the European Research Council grant agreement ERC-2014-ADG-669858 (ATLAS). J. Borges gratefully acknowledges FCT for the individual contract (CEECIND/ 03202/2017). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Foundation for Science and Technology/MCTES. Publisher Copyright: © The Royal Society of Chemistry.

PY - 2020/7/14

Y1 - 2020/7/14

N2 - The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.

AB - The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.

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U2 - 10.1039/d0sm00876a

DO - 10.1039/d0sm00876a

M3 - Article

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AN - SCOPUS:85088211200

SN - 1744-683X

VL - 16

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JO - Soft Matter

JF - Soft Matter

IS - 26

ER -

Enzymatically degradable, starch-based layer-by-layer films: Application to cytocompatible single-cell nanoencapsulation

Abstract

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