Enzymatic synthesis of new hepoxilins and trioxilins from polyunsaturated fatty acids

In Gyu Lee, Jung Ung An, Yoon Joo Ko, Jin Byung Park, Deok Kun Oh

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Hepoxilins (HXs) and trioxilins (TrXs) are lipid mediators that regulate diverse physiological processes at trace amounts in humans. They have been synthesized by chemical methods, which have disadvantages such as low yield, byproduct formation, and use of toxic chemical reagents. Here, we reported the identification of ten types of HXs and TrXs synthesized by recombinant Escherichia coli expressing bacterial arachidonate 15-lipoxygenase and/or epoxide hydrolase using five polyunsaturated fatty acids (PUFAs). Of these, eight types were new. The discovery was due to the microbial enzymes with high activities for the same catalytic activities as those of human enzymes. We succeeded in the efficient, cost-effective, and environmentally friendly biotransformation of PUFAs into nine types of HXs and TrXs by cells, yielding >10 g L-1 of 14,15-HXB3 and 13,14,15-TrXB3, with conversion yields of >70%. Our study contributes to the environmentally friendly synthesis of medicinally important lipid mediators.

Original languageEnglish
Pages (from-to)3172-3181
Number of pages10
JournalGreen Chemistry
Volume21
Issue number11
DOIs
StatePublished - 2019

Bibliographical note

Funding Information:
This study was funded by the Mid-Career Researcher Program, through the National Research Foundation grant funded by the Ministry of Science and ICT, Republic of Korea (No. 2019R1A2C3002781).

Publisher Copyright:
© 2019 The Royal Society of Chemistry.

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