Enzymatic in vitro glycosylation using peptide-N-glycosidase F

Ji Youn Lee, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


A high yield and cost effective process for the production of glycoproteins is attainable based on a low cost prokaryotic system with a high yield production of a nonglycosylated form, which is then glycosylated in vitro. Accordingly, the enzymatic deglycosylation of glycoproteins and enzymatic glycosylation of deglycosylated proteins were investigated using peptide-N-glycosidase F. Enzymatic in vitro glycosylation was found to occur with excess maltodextrin and ammonium salt in acetone at 50°C.

Original languageEnglish
Pages (from-to)716-720
Number of pages5
JournalEnzyme and Microbial Technology
Issue number6
StatePublished - 27 May 2002

Bibliographical note

Funding Information:
The authors wish to acknowledge the financial support of LG Chemical Co. and the Nano Bio-Electronic & System Center.


  • Deglycosylation
  • Glycosylation
  • Hydrazinolysis
  • Organic solvent
  • Peptide-N-glycosidase F


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