Enhancement of phase separation by the addition of de-emulsifiers to three-phase (diesel oil/biocatalyst/aqueous phase) emulsion in diesel biodesulfurization

Kyoung Choi Ok; Suk Cho Kyung; Wook Ryu Hee; Keun Chang Yong

doi:10.1023/A:1021750719337

Enhancement of phase separation by the addition of de-emulsifiers to three-phase (diesel oil/biocatalyst/aqueous phase) emulsion in diesel biodesulfurization

Kyoung Choi Ok, Suk Cho Kyung, Wook Ryu Hee, Keun Chang Yong

Department of Environmental Science & Engineering

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocatalyst recovered with ethanol addition showed similar specific growth rates (0.03 h^-1) and dibenzothiophene desulfurization rates (6-7.2 mol 1^-1 h^-1) to those (0.03 h^-1 and 7.1 mol 1^-1, respectively) of the biocatalyst recovered with no ethanol addition. The desulfurization activity significantly increased as the number of the repeated recovery and reuse of the biocatalyst.

Original language	English
Pages (from-to)	73-77
Number of pages	5
Journal	Biotechnology Letters
Volume	25
Issue number	1
DOIs	https://doi.org/10.1023/A:1021750719337
State	Published - Jan 2003

Bibliographical note

Funding Information:
This work was supported by Clean Energy Program Grant, Ministry of Commerce, Industry and Resources, and R&D Management Center for Energy and Resources, Korea.

Keywords

Biodesulfurization
De-emulsifiers
Gordonia nitida
Phase separation

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title = "Enhancement of phase separation by the addition of de-emulsifiers to three-phase (diesel oil/biocatalyst/aqueous phase) emulsion in diesel biodesulfurization",

abstract = "Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocatalyst recovered with ethanol addition showed similar specific growth rates (0.03 h-1) and dibenzothiophene desulfurization rates (6-7.2 mol 1-1 h-1) to those (0.03 h-1 and 7.1 mol 1-1, respectively) of the biocatalyst recovered with no ethanol addition. The desulfurization activity significantly increased as the number of the repeated recovery and reuse of the biocatalyst.",

keywords = "Biodesulfurization, De-emulsifiers, Gordonia nitida, Phase separation",

author = "Ok, {Kyoung Choi} and Kyung, {Suk Cho} and Hee, {Wook Ryu} and Yong, {Keun Chang}",

note = "Funding Information: This work was supported by Clean Energy Program Grant, Ministry of Commerce, Industry and Resources, and R&D Management Center for Energy and Resources, Korea.",

year = "2003",

month = jan,

doi = "10.1023/A:1021750719337",

language = "English",

volume = "25",

pages = "73--77",

journal = "Biotechnology Letters",

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T1 - Enhancement of phase separation by the addition of de-emulsifiers to three-phase (diesel oil/biocatalyst/aqueous phase) emulsion in diesel biodesulfurization

AU - Ok, Kyoung Choi

AU - Kyung, Suk Cho

AU - Hee, Wook Ryu

AU - Yong, Keun Chang

N1 - Funding Information: This work was supported by Clean Energy Program Grant, Ministry of Commerce, Industry and Resources, and R&D Management Center for Energy and Resources, Korea.

PY - 2003/1

Y1 - 2003/1

N2 - Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocatalyst recovered with ethanol addition showed similar specific growth rates (0.03 h-1) and dibenzothiophene desulfurization rates (6-7.2 mol 1-1 h-1) to those (0.03 h-1 and 7.1 mol 1-1, respectively) of the biocatalyst recovered with no ethanol addition. The desulfurization activity significantly increased as the number of the repeated recovery and reuse of the biocatalyst.

AB - Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocatalyst recovered with ethanol addition showed similar specific growth rates (0.03 h-1) and dibenzothiophene desulfurization rates (6-7.2 mol 1-1 h-1) to those (0.03 h-1 and 7.1 mol 1-1, respectively) of the biocatalyst recovered with no ethanol addition. The desulfurization activity significantly increased as the number of the repeated recovery and reuse of the biocatalyst.

KW - Biodesulfurization

KW - De-emulsifiers

KW - Gordonia nitida

KW - Phase separation

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U2 - 10.1023/A:1021750719337

DO - 10.1023/A:1021750719337

M3 - Article

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AN - SCOPUS:0037262563

SN - 0141-5492

VL - 25

SP - 73

EP - 77

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 1

ER -

Enhancement of phase separation by the addition of de-emulsifiers to three-phase (diesel oil/biocatalyst/aqueous phase) emulsion in diesel biodesulfurization

Abstract

Bibliographical note

Keywords

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