Enhanced production of recombinant protein in Escherichia coli using silkworm hemolymph

Ji Eun Kim, Eun Jeong Kim, Won Jong Rhee, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The effect of silkworm hemolymph on the expression of recombinant protein in Escherichia coli was investigated. The addition of silkworm hemolymph to the culture medium increased the production of recombinant β-galactosidase in E. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in media supplemented with 1, 3, and 5% silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified as the effective component.

Original languageEnglish
Pages (from-to)353-356
Number of pages4
JournalBiotechnology and Bioprocess Engineering
Issue number4
StatePublished - 2005

Bibliographical note

Funding Information:
^ÅâåçïäÉÇÖãÉåí The authors wish to acknowledge the financial support of the Korea Science & Engineering Foundation through the Nano Bio-Electronic & System Center.


  • E. coli
  • Recombinant protein
  • Silkworm hemolymph


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