Enhanced production of recombinant protein in Escherichia coli using silkworm hemolymph

Ji Eun Kim; Eun Jeong Kim; Won Jong Rhee; Tai Hyun Park

doi:10.1007/BF02931854

Enhanced production of recombinant protein in Escherichia coli using silkworm hemolymph

Ji Eun Kim, Eun Jeong Kim, Won Jong Rhee, Tai Hyun Park

Department of Nutritional Science & Food Management

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The effect of silkworm hemolymph on the expression of recombinant protein in Escherichia coli was investigated. The addition of silkworm hemolymph to the culture medium increased the production of recombinant β-galactosidase in E. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in media supplemented with 1, 3, and 5% silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified as the effective component.

Original language	English
Pages (from-to)	353-356
Number of pages	4
Journal	Biotechnology and Bioprocess Engineering
Volume	10
Issue number	4
DOIs	https://doi.org/10.1007/BF02931854
State	Published - 2005

Bibliographical note

Funding Information:
^ÅâåçïäÉÇÖãÉåí The authors wish to acknowledge the financial support of the Korea Science & Engineering Foundation through the Nano Bio-Electronic & System Center.

Keywords

E. coli
Recombinant protein
Silkworm hemolymph

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10.1007/BF02931854

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title = "Enhanced production of recombinant protein in Escherichia coli using silkworm hemolymph",

abstract = "The effect of silkworm hemolymph on the expression of recombinant protein in Escherichia coli was investigated. The addition of silkworm hemolymph to the culture medium increased the production of recombinant β-galactosidase in E. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in media supplemented with 1, 3, and 5% silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified as the effective component.",

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year = "2005",

doi = "10.1007/BF02931854",

language = "English",

volume = "10",

pages = "353--356",

journal = "Biotechnology and Bioprocess Engineering",

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publisher = "Korean Society for Biotechnology and Bioengineering",

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T1 - Enhanced production of recombinant protein in Escherichia coli using silkworm hemolymph

AU - Kim, Ji Eun

AU - Kim, Eun Jeong

AU - Rhee, Won Jong

AU - Park, Tai Hyun

N1 - Funding Information: ^ÅâåçïäÉÇÖãÉåí The authors wish to acknowledge the financial support of the Korea Science & Engineering Foundation through the Nano Bio-Electronic & System Center.

PY - 2005

Y1 - 2005

N2 - The effect of silkworm hemolymph on the expression of recombinant protein in Escherichia coli was investigated. The addition of silkworm hemolymph to the culture medium increased the production of recombinant β-galactosidase in E. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in media supplemented with 1, 3, and 5% silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified as the effective component.

AB - The effect of silkworm hemolymph on the expression of recombinant protein in Escherichia coli was investigated. The addition of silkworm hemolymph to the culture medium increased the production of recombinant β-galactosidase in E. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in media supplemented with 1, 3, and 5% silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified as the effective component.

KW - E. coli

KW - Recombinant protein

KW - Silkworm hemolymph

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U2 - 10.1007/BF02931854

DO - 10.1007/BF02931854

M3 - Article

AN - SCOPUS:24344431534

SN - 1226-8372

VL - 10

SP - 353

EP - 356

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 4

ER -

Enhanced production of recombinant protein in Escherichia coli using silkworm hemolymph

Abstract

Bibliographical note

Keywords

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