Abstract
Whole-cell conversion of cyclohexanone to ε-caprolactone was attempted by recombinant Escherichia coli BL21(DE3) expressing cyclohexanone monooxygenase (CHMO) of Acinetobacter calcoaceticus NCIMB 9871. High concentrations of cyclohexanone and ε-caprolactone reduced CHMO-mediated bioconversion of cyclohexanone to ε-caprolactone in the resting recombinant E. coli cells. Metabolically active cells were employed by adopting a fed-batch culture to improve the production of ε-caprolactone from cyclohexanone. A glucose-limited fed-batch Baeyer-Villiger oxidation where a cyclohexanone level was maintained less than 6 g/l resulted in a maximum ε-caprolactone concentration of 11.0 g/l. The maximum ε-caprolactone concentration was improved further to 15.3 g/l by coexpression of glucose-6-phosphate dehydrogenase, an NADPH-generating enzyme encoded by the zwf gene which corresponded to a 39% enhancement in ε-caprolactone concentration compared with the control experiment performed under the same conditions.
Original language | English |
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Pages (from-to) | 329-338 |
Number of pages | 10 |
Journal | Applied Microbiology and Biotechnology |
Volume | 76 |
Issue number | 2 |
DOIs | |
State | Published - Aug 2007 |
Keywords
- Cyclohexanone monooxygenase
- Escherichia coli
- Fed-batch
- Glucose 6-phosphate dehydrogenase
- NADPH
- Substrate/product inhibition