Enhanced isobutanol production from acetate by combinatorial overexpression of acetyl-CoA synthetase and anaplerotic enzymes in engineered Escherichia coli

Hun Suk Song, Hyung Min Seo, Jong Min Jeon, Yu Mi Moon, Ju Won Hong, Yoon Gi Hong, Shashi Kant Bhatia, Jungoh Ahn, Hongweon Lee, Wooseong Kim, Yong Cheol Park, Kwon Young Choi, Yun Gon Kim, Yung Hun Yang

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Acetic acid is an abundant material that can be used as a carbon source by microorganisms. Despite its abundance, its toxicity and low energy content make it hard to utilize as a sole carbon source for biochemical production. To increase acetate utilization and isobutanol production with engineered Escherichia coli, the feasibility of utilizing acetate and metabolic engineering was investigated. The expression of acs, pckA, and maeB increased isobutanol production by up to 26%, and the addition of TCA cycle intermediates indicated that the intermediates can enhance isobutanol production. For isobutanol production from acetate, acetate uptake rates and the NADPH pool were not limiting factors compared to glucose as a carbon source. This work represents the first approach to produce isobutanol from acetate with pyruvate flux optimization to extend the applicability of acetate. This technique suggests a strategy for biochemical production utilizing acetate as the sole carbon source.

Original languageEnglish
Pages (from-to)1971-1978
Number of pages8
JournalBiotechnology and Bioengineering
Volume115
Issue number8
DOIs
StatePublished - Aug 2018

Keywords

  • acetate
  • anaplerotic enzymes
  • Escherichia coli
  • isobutanol

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