Enhanced intracellular delivery of macromolecules by melittin derivatives mediated cellular uptake

Heiwon Kyung; Heejung Kim; Hyukjin Lee; Seung Jin Lee

doi:10.1016/j.jiec.2017.09.039

Enhanced intracellular delivery of macromolecules by melittin derivatives mediated cellular uptake

Heiwon Kyung, Heejung Kim, Hyukjin Lee, Seung Jin Lee

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Various cell-penetrating peptides (CPPs) have been utilized as a promising tool for intracellular delivery of therapeutic macromolecules. In this study, hemolytic membrane-active peptide (Melittin) has been utilized for intracellular uptake of macromolecules by its sequential fragmentation. Among its fragmented derivatives, melittin(1-14) showed highly efficient delivery of proteins into HeLa cells, while maintaining their biological activities. The highest level of intracellular delivery was observed when ß-galactosidase and Cytochrome C were co-delivered with melittin(1-14). These data suggested that melittin(1-14) could be a useful adjuvant for the enhanced intracellular delivery of therapeutic macromolecules.

Original language	English
Pages (from-to)	290-295
Number of pages	6
Journal	Journal of Industrial and Engineering Chemistry
Volume	58
DOIs	https://doi.org/10.1016/j.jiec.2017.09.039
State	Published - 25 Feb 2018

Bibliographical note

Funding Information:
This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) & funded by the Korean government (MSIP&MOHW) ( 2014M3C1A3054153 , 2015M3A9B4066662 and 2016K1A3A1A20006058 )

Publisher Copyright:
© 2017

Keywords

Cell-penetrating peptide
Intracellular delivery
Melittin

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10.1016/j.jiec.2017.09.039

Cite this

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title = "Enhanced intracellular delivery of macromolecules by melittin derivatives mediated cellular uptake",

abstract = "Various cell-penetrating peptides (CPPs) have been utilized as a promising tool for intracellular delivery of therapeutic macromolecules. In this study, hemolytic membrane-active peptide (Melittin) has been utilized for intracellular uptake of macromolecules by its sequential fragmentation. Among its fragmented derivatives, melittin(1-14) showed highly efficient delivery of proteins into HeLa cells, while maintaining their biological activities. The highest level of intracellular delivery was observed when {\ss}-galactosidase and Cytochrome C were co-delivered with melittin(1-14). These data suggested that melittin(1-14) could be a useful adjuvant for the enhanced intracellular delivery of therapeutic macromolecules.",

keywords = "Cell-penetrating peptide, Intracellular delivery, Melittin",

author = "Heiwon Kyung and Heejung Kim and Hyukjin Lee and Lee, \{Seung Jin\}",

note = "Funding Information: This research was supported by the Bio \& Medical Technology Development Program of the National Research Foundation (NRF) \& funded by the Korean government (MSIP\&MOHW) ( 2014M3C1A3054153 , 2015M3A9B4066662 and 2016K1A3A1A20006058 ) Publisher Copyright: {\textcopyright} 2017",

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doi = "10.1016/j.jiec.2017.09.039",

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journal = "Journal of Industrial and Engineering Chemistry",

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T1 - Enhanced intracellular delivery of macromolecules by melittin derivatives mediated cellular uptake

AU - Kyung, Heiwon

AU - Kim, Heejung

AU - Lee, Hyukjin

AU - Lee, Seung Jin

N1 - Funding Information: This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) & funded by the Korean government (MSIP&MOHW) ( 2014M3C1A3054153 , 2015M3A9B4066662 and 2016K1A3A1A20006058 ) Publisher Copyright: © 2017

PY - 2018/2/25

Y1 - 2018/2/25

N2 - Various cell-penetrating peptides (CPPs) have been utilized as a promising tool for intracellular delivery of therapeutic macromolecules. In this study, hemolytic membrane-active peptide (Melittin) has been utilized for intracellular uptake of macromolecules by its sequential fragmentation. Among its fragmented derivatives, melittin(1-14) showed highly efficient delivery of proteins into HeLa cells, while maintaining their biological activities. The highest level of intracellular delivery was observed when ß-galactosidase and Cytochrome C were co-delivered with melittin(1-14). These data suggested that melittin(1-14) could be a useful adjuvant for the enhanced intracellular delivery of therapeutic macromolecules.

AB - Various cell-penetrating peptides (CPPs) have been utilized as a promising tool for intracellular delivery of therapeutic macromolecules. In this study, hemolytic membrane-active peptide (Melittin) has been utilized for intracellular uptake of macromolecules by its sequential fragmentation. Among its fragmented derivatives, melittin(1-14) showed highly efficient delivery of proteins into HeLa cells, while maintaining their biological activities. The highest level of intracellular delivery was observed when ß-galactosidase and Cytochrome C were co-delivered with melittin(1-14). These data suggested that melittin(1-14) could be a useful adjuvant for the enhanced intracellular delivery of therapeutic macromolecules.

KW - Cell-penetrating peptide

KW - Intracellular delivery

KW - Melittin

UR - https://www.scopus.com/pages/publications/85033224155

U2 - 10.1016/j.jiec.2017.09.039

DO - 10.1016/j.jiec.2017.09.039

M3 - Article

AN - SCOPUS:85033224155

SN - 1226-086X

VL - 58

SP - 290

EP - 295

JO - Journal of Industrial and Engineering Chemistry

JF - Journal of Industrial and Engineering Chemistry

ER -

Enhanced intracellular delivery of macromolecules by melittin derivatives mediated cellular uptake

Abstract

Bibliographical note

Keywords

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