Enhanced intracellular delivery of macromolecules by melittin derivatives mediated cellular uptake

Heiwon Kyung, Heejung Kim, Hyukjin Lee, Seung Jin Lee

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Various cell-penetrating peptides (CPPs) have been utilized as a promising tool for intracellular delivery of therapeutic macromolecules. In this study, hemolytic membrane-active peptide (Melittin) has been utilized for intracellular uptake of macromolecules by its sequential fragmentation. Among its fragmented derivatives, melittin(1-14) showed highly efficient delivery of proteins into HeLa cells, while maintaining their biological activities. The highest level of intracellular delivery was observed when ß-galactosidase and Cytochrome C were co-delivered with melittin(1-14). These data suggested that melittin(1-14) could be a useful adjuvant for the enhanced intracellular delivery of therapeutic macromolecules.

Original languageEnglish
Pages (from-to)290-295
Number of pages6
JournalJournal of Industrial and Engineering Chemistry
Volume58
DOIs
StatePublished - 25 Feb 2018

Keywords

  • Cell-penetrating peptide
  • Intracellular delivery
  • Melittin

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