Abstract
Various cell-penetrating peptides (CPPs) have been utilized as a promising tool for intracellular delivery of therapeutic macromolecules. In this study, hemolytic membrane-active peptide (Melittin) has been utilized for intracellular uptake of macromolecules by its sequential fragmentation. Among its fragmented derivatives, melittin(1-14) showed highly efficient delivery of proteins into HeLa cells, while maintaining their biological activities. The highest level of intracellular delivery was observed when ß-galactosidase and Cytochrome C were co-delivered with melittin(1-14). These data suggested that melittin(1-14) could be a useful adjuvant for the enhanced intracellular delivery of therapeutic macromolecules.
Original language | English |
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Pages (from-to) | 290-295 |
Number of pages | 6 |
Journal | Journal of Industrial and Engineering Chemistry |
Volume | 58 |
DOIs | |
State | Published - 25 Feb 2018 |
Keywords
- Cell-penetrating peptide
- Intracellular delivery
- Melittin