Enhanced cellular uptake of silica-coated magnetite nanoparticles compared with PEG-coated ones in stem cells

Dong Heon Lee, Myunggoo Kang, Hong Jai Lee, Jeong Ah Kim, Yun Kyong Choi, Hyunjin Cho, Jung Keug Park, Tai Hyun Park, Hyun Jung

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Monodispersed magnetite (Fe3O4) nanoparticles (NPs) were prepared through the thermal decomposition method. The obtained NPs were surface modified with silica (SiO2) and polyethylene glycol (PEG), to enhance their stability in aqueous environment and their cellular uptake efficiency for biomedical applications. The NPs were characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), Fourier transform infrared (FT-IR) spectroscopy, and dynamic light scattering (DLS). The cytotoxicity of these NPs on bone marrow mesenchymal stem cells (BM-MSCs) was measured by MTT assay (cell viability test) at various concentrations (2, 5, 12.5, 25, and 50 μg/mL). The cells remained more than 90% viable at concentrations as high as 50 μg/mL. To compare the cellular uptake efficiency, these NPs were treated in BM-MSCs and the Fe concentration within the cells was measured by inductively coupled plasma-atomic emission spectrometry (ICP-AES) analysis. The uptake process displayed a time- and dose-dependency. The uptake amount of SiO2-coated Fe3O4 (Fe3O4@SiO2) NPs was about 10 times higher than that of the PEG-coated ones (Fe3O4@PEG).

Original languageEnglish
Pages (from-to)5512-5519
Number of pages8
JournalJournal of Nanoscience and Nanotechnology
Issue number8
StatePublished - 10 Jan 2015

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Copyright © 2015 American Scientific Publishers. All rights reserved.


  • Bone marrow mesenchymal stem cells
  • Cellular uptake
  • Magnetic nanoparticles
  • Surface modification


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