Engineering the xylose-catabolizing Dahms pathway for production of poly(d-lactate-co-glycolate) and poly(d-lactate-co-glycolate-co-d-2-hydroxybutyrate) in Escherichia coli

So Young Choi, Won Jun Kim, Seung Jung Yu, Si Jae Park, Sung Gap Im, Sang Yup Lee

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Poly(lactate-co-glycolate), PLGA, is a representative synthetic biopolymer widely used in medical applications. Recently, we reported one-step direct fermentative production of PLGA and its copolymers by metabolically engineered Escherichia coli from xylose and glucose. In this study, we report development of metabolically engineered E. coli strains for the production of PLGA and poly(d-lactate-co-glycolate-co-d-2-hydroxybutyrate) having various monomer compositions from xylose as a sole carbon source. To achieve this, the metabolic flux towards Dahms pathway was modulated using five different synthetic promoters for the expression of Caulobacter crescentus XylBC. Further metabolic engineering to concentrate the metabolic flux towards d-lactate and glycolate resulted in production of PLGA and poly(d-lactate-co-glycolate-co-d-2-hydroxybutyrate) with various monomer fractions from xylose. The engineered E. coli strains produced polymers containing 8.8–60.9 mol% of glycolate up to 6.93 g l−1 by fed-batch cultivation in a chemically defined medium containing xylose. Finally, the biocompatibility of poly(d-lactate-co-glycolate-co-d-2-hydroxybutyrate) was confirmed by live/dead assay using human mesenchymal stem cells.

Original languageEnglish
Pages (from-to)1353-1364
Number of pages12
JournalMicrobial Biotechnology
Volume10
Issue number6
DOIs
StatePublished - Nov 2017

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