Engineering of a bacterial outer membrane vesicle to a nano-scale reactor for the biodegradation of β-lactam antibiotics

Ji Min Woo, Myeong Yeon Kim, Ji Won Song, Yoonjin Baeg, Hye Jin Jo, Sun Shin Cha, Jin Byung Park

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, which are involved in various functions including cell to cell signaling and protein excretion. Here, we have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the degradation of β-lactam antibiotics. This was exploited by targeting a β-lactamase (i.e., CMY-10) into the OMVs of a hyper-vesiculating E. coli BL21(DE3) mutant. The CMY-10-containing OMVs, prepared from the E. coli mutant cultures, were able to hydrolyze β-lactam ring of nitrocefin and meropenem to a specific rate of 6.6 × 10-8 and 3.9 × 10-12 μmol/min/µm3 of OMV, which is approximately 100 and 600-fold greater than those of E. coli-based whole-cell biocatalsyts. Furthermore, CMY-10, which was encapsulated in the engineered OMVs, was much more stable against temperature and acid stresses, as compared to free enzymes in aqueous phase. The OMV-based nano-scaled reaction system would be useful for the remediation of a variety of antibiotics pollution for food and agricultural industry.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Biotechnology
StatePublished - 10 Sep 2022

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government (MEST) (No. 2020R1A2B5B03002376 ) and by the project titled “ Development of potential antibiotic compounds using polar organism resources ( 15250103 , KOPRI Grant PM21030 )”, funded by the Ministry of Oceans and Fisheries (MOF), Korea. J. M. Woo was supported by RP-Grant of Ewha Womans University and BK21 program.

Publisher Copyright:
© 2022


  • Antibiotics
  • CMY-10
  • Nano-scale bioreactor
  • Outer membrane vesicles


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