TY - JOUR
T1 - Engineering large-scale hiPSC-derived vessel-integrated muscle-like lattices for enhanced volumetric muscle regeneration
AU - Lee, Myung Chul
AU - Jodat, Yasamin A.
AU - Endo, Yori
AU - Rodríguez-delaRosa, Alejandra
AU - Zhang, Ting
AU - Karvar, Mehran
AU - Al Tanoury, Ziad
AU - Quint, Jacob
AU - Kamperman, Tom
AU - Kiaee, Kiavash
AU - Ochoa, Sofia Lara
AU - Shi, Kun
AU - Huang, Yike
AU - Rosales, Montserrat Pineda
AU - Arnaout, Adnan
AU - Lee, Hyeseon
AU - Kim, Jiseong
AU - Ceron, Eder Luna
AU - Reyes, Isaac Garcia
AU - Panayi, Adriana C.
AU - Martinez, Angel Flores Huidobro
AU - Wang, Xichi
AU - Kim, Ki Tae
AU - Moon, Jae I.
AU - Park, Seung Gwa
AU - Lee, Kangju
AU - Calabrese, Michelle A.
AU - Hassan, Shabir
AU - Lee, Junmin
AU - Tamayol, Ali
AU - Lee, Luke
AU - Pourquié, Olivier
AU - Kim, Woo Jin
AU - Sinha, Indranil
AU - Shin, Su Ryon
N1 - Publisher Copyright:
© 2024 Elsevier Ltd
PY - 2024/12
Y1 - 2024/12
N2 - Engineering biomimetic tissue implants with human induced pluripotent stem cells (hiPSCs) holds promise for repairing volumetric tissue loss. However, these implants face challenges in regenerative capability, survival, and geometric scalability at large-scale injury sites. Here, we present scalable vessel-integrated muscle-like lattices (VMLs), containing dense and aligned hiPSC-derived myofibers alongside passively perfusable vessel-like microchannels inside an endomysium-like supporting matrix using an embedded multimaterial bioprinting technology. The contractile and millimeter-long myofibers are created in mechanically tailored and nanofibrous extracellular matrix-based hydrogels. Incorporating vessel-like lattice enhances myofiber maturation in vitro and guides host vessel invasion in vivo, improving implant integration. Consequently, we demonstrate successful de novo muscle formation and muscle function restoration through a combinatorial effect between improved graft–host integration and its increased release of paracrine factors within volumetric muscle loss injury models. The proposed modular bioprinting technology enables scaling up to centimeter-sized prevascularized hiPSC-derived muscle tissues with custom geometries for next-generation muscle regenerative therapies.
AB - Engineering biomimetic tissue implants with human induced pluripotent stem cells (hiPSCs) holds promise for repairing volumetric tissue loss. However, these implants face challenges in regenerative capability, survival, and geometric scalability at large-scale injury sites. Here, we present scalable vessel-integrated muscle-like lattices (VMLs), containing dense and aligned hiPSC-derived myofibers alongside passively perfusable vessel-like microchannels inside an endomysium-like supporting matrix using an embedded multimaterial bioprinting technology. The contractile and millimeter-long myofibers are created in mechanically tailored and nanofibrous extracellular matrix-based hydrogels. Incorporating vessel-like lattice enhances myofiber maturation in vitro and guides host vessel invasion in vivo, improving implant integration. Consequently, we demonstrate successful de novo muscle formation and muscle function restoration through a combinatorial effect between improved graft–host integration and its increased release of paracrine factors within volumetric muscle loss injury models. The proposed modular bioprinting technology enables scaling up to centimeter-sized prevascularized hiPSC-derived muscle tissues with custom geometries for next-generation muscle regenerative therapies.
KW - bioprinting
KW - engineering vascularized tissues
KW - induced pluripotent stem cells
KW - secreted factors, volumetric muscle loss
KW - skeletal muscle engineering
UR - http://www.scopus.com/inward/record.url?scp=85204478348&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2024.08.001
DO - 10.1016/j.tibtech.2024.08.001
M3 - Article
C2 - 39306493
AN - SCOPUS:85204478348
SN - 0167-7799
VL - 42
SP - 1715
EP - 1744
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 12
ER -