Two-component regulatory systems (TCRSs) mediate cellular response by coupling sensing and regulatory mechanisms. TCRSs are comprised of a histidine kinase (HK), which serves as a sensor, and a response regulator, which regulates expression of the effector gene after being phosphorylated by HK. Using these attributes, bacterial TCRSs can be engineered to design microbial systems for different applications. This review focuses on the current advances in TCRS-based biosensors and on the design of microbial systems for bioremediation and their potential application in biorefinery.
Bibliographical noteFunding Information:
This work was supported by a grant by the National Research Founda‑ tion of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (MSIP) (NRF‑2015R1A2A2A01004733), a Golden Seed Project Grant (213008‑05‑1‑SB910) funded by Ministry of Oceans and Fisheries, and the Mid‑career Researcher Program from MSIP through NRF of Korea (NRF‑2016R1A2B4008707).
© 2017 The Author(s).
- Genetic circuit
- Two-component regulatory system