Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery

Sambandam Ravikumar; Mary Grace Baylon; Si Jae Park; Jong il Choi

doi:10.1186/s12934-017-0675-z

Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery

Sambandam Ravikumar, Mary Grace Baylon, Si Jae Park, Jong il Choi

Chemical Engineering & Materials Science

Research output: Contribution to journal › Review article › peer-review

49 Scopus citations

Abstract

Two-component regulatory systems (TCRSs) mediate cellular response by coupling sensing and regulatory mechanisms. TCRSs are comprised of a histidine kinase (HK), which serves as a sensor, and a response regulator, which regulates expression of the effector gene after being phosphorylated by HK. Using these attributes, bacterial TCRSs can be engineered to design microbial systems for different applications. This review focuses on the current advances in TCRS-based biosensors and on the design of microbial systems for bioremediation and their potential application in biorefinery.

Original language	English
Article number	62
Journal	Microbial Cell Factories
Volume	16
Issue number	1
DOIs	https://doi.org/10.1186/s12934-017-0675-z
State	Published - 14 Apr 2017

Bibliographical note

Funding Information:
This work was supported by a grant by the National Research Founda‑ tion of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (MSIP) (NRF‑2015R1A2A2A01004733), a Golden Seed Project Grant (213008‑05‑1‑SB910) funded by Ministry of Oceans and Fisheries, and the Mid‑career Researcher Program from MSIP through NRF of Korea (NRF‑2016R1A2B4008707).

Publisher Copyright:
© 2017 The Author(s).

Keywords

Biorefinery
Bioremediation
Biosensor
Genetic circuit
Two-component regulatory system

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10.1186/s12934-017-0675-z

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title = "Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery",

abstract = "Two-component regulatory systems (TCRSs) mediate cellular response by coupling sensing and regulatory mechanisms. TCRSs are comprised of a histidine kinase (HK), which serves as a sensor, and a response regulator, which regulates expression of the effector gene after being phosphorylated by HK. Using these attributes, bacterial TCRSs can be engineered to design microbial systems for different applications. This review focuses on the current advances in TCRS-based biosensors and on the design of microbial systems for bioremediation and their potential application in biorefinery.",

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AU - Baylon, Mary Grace

AU - Park, Si Jae

AU - Choi, Jong il

N1 - Funding Information: This work was supported by a grant by the National Research Founda‑ tion of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (MSIP) (NRF‑2015R1A2A2A01004733), a Golden Seed Project Grant (213008‑05‑1‑SB910) funded by Ministry of Oceans and Fisheries, and the Mid‑career Researcher Program from MSIP through NRF of Korea (NRF‑2016R1A2B4008707). Publisher Copyright: © 2017 The Author(s).

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AB - Two-component regulatory systems (TCRSs) mediate cellular response by coupling sensing and regulatory mechanisms. TCRSs are comprised of a histidine kinase (HK), which serves as a sensor, and a response regulator, which regulates expression of the effector gene after being phosphorylated by HK. Using these attributes, bacterial TCRSs can be engineered to design microbial systems for different applications. This review focuses on the current advances in TCRS-based biosensors and on the design of microbial systems for bioremediation and their potential application in biorefinery.

KW - Biorefinery

KW - Bioremediation

KW - Biosensor

KW - Genetic circuit

KW - Two-component regulatory system

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SN - 1475-2859

VL - 16

JO - Microbial Cell Factories

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ER -

Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery

Abstract

Bibliographical note

Keywords

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