Engineered lentivector targeting of dendritic cells for in vivo immunization

Lili Yang, Haiguang Yang, Kendra Rideout, Taehoon Cho, Kye Il Joo, Leslie Ziegler, Abigail Elliot, Anthony Walls, Dongzi Yu, David Baltimore, Pin Wang

Research output: Contribution to journalArticlepeer-review

168 Scopus citations

Abstract

We report a method of inducing antigen production in dendritic cells by in vivo targeting with lentiviral vectors that specifically bind to the dendritic cell-surface protein DC-SIGN. To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to be DC-SIGN-specific. In vitro, this lentivector specifically transduced dendritic cells and induced dendritic cell maturation. A high frequency (up to 12%) of ovalbumin (OVA)-specific CD8+ T cells and a significant antibody response were observed 2 weeks after injection of a targeted lentiviral vector encoding an OVA transgene into naive mice. This approach also protected against the growth of OVA-expressing E.G7 tumors and induced regression of established tumors. Thus, lentiviral vectors targeting dendritic cells provide a simple method of producing effective immunity and may provide an alternative route for immunization with protein antigens.

Original languageEnglish
Pages (from-to)326-334
Number of pages9
JournalNature Biotechnology
Volume26
Issue number3
DOIs
StatePublished - Mar 2008

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