Engineered lentivector targeting of dendritic cells for in vivo immunization

Lili Yang, Haiguang Yang, Kendra Rideout, Taehoon Cho, Kye Il Joo, Leslie Ziegler, Abigail Elliot, Anthony Walls, Dongzi Yu, David Baltimore, Pin Wang

Research output: Contribution to journalArticlepeer-review

174 Scopus citations


We report a method of inducing antigen production in dendritic cells by in vivo targeting with lentiviral vectors that specifically bind to the dendritic cell-surface protein DC-SIGN. To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to be DC-SIGN-specific. In vitro, this lentivector specifically transduced dendritic cells and induced dendritic cell maturation. A high frequency (up to 12%) of ovalbumin (OVA)-specific CD8+ T cells and a significant antibody response were observed 2 weeks after injection of a targeted lentiviral vector encoding an OVA transgene into naive mice. This approach also protected against the growth of OVA-expressing E.G7 tumors and induced regression of established tumors. Thus, lentiviral vectors targeting dendritic cells provide a simple method of producing effective immunity and may provide an alternative route for immunization with protein antigens.

Original languageEnglish
Pages (from-to)326-334
Number of pages9
JournalNature Biotechnology
Issue number3
StatePublished - Mar 2008

Bibliographical note

Funding Information:
We are grateful to James Strauss for providing reagents and April Tai for a critical reading of the manuscript. This work was supported by a grant from the National Institute of Health (R01AI068978), a grant from the Bill and Melinda Gates Foundation through the Grand Challenges in Global Health Initiative, and by the Skirball Foundation.


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