Endothelial nitric oxide synthase gene is associated with vessel stenosis in Korean population

Suin Yoon, Chol Shin, Hyun Young Park, Jesung Moon, Eunkyung Kim, Heung Tae Kim, Jiho Min, Sangmee Ahn Jo, Inho Jo

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Background: Nitric oxide (NO) produced by endothelial NO synthase (eNOS) mediates endothelium-dependent vasodilation and antithrombotic action. Controversial results regarding the association of eNOS gene polymorphisms with vessel stenosis have been reported. Methods: Age- and sex-matched 932 individuals (656 subjects having 1-, 2-, and 3-stenosed vessels and 276 controls without stenosis) living in Seoul and surrounding suburbs were selected. A GT missense mutation in exon 7 (894GT) was screened using PCR-restriction fragment length polymorphism analysis. The genotypes of a 27-bp insertion/deletion in intron 4 (eNOS4b/a) and a TC mutation in promoter region of -786 (-786TC) were determined by the banding pattern on gel electrophoresis and a commercially available minisequencing protocol (SNaPshot), respectively. Results: The eNOS4a allele was highly linked to the -786C allele (r=0.93, P<0.0001) while there was no linkage between eNOS4a allele and 894T allele or between 894T allele and -786C allele. Furthermore, 894T allele, but not eNOS4a (-786C) allele, was associated with the presence, but not the number, of stenosed vessels (odds ratio=1.57 for dominant effect of the T allele, P<0.05, and 1.49 for additive effect, P<0.05). Multiple logistic regression analysis revealed that 894T allele and hypertension were predictive independent risk factors for the presence of vessel stenosis. Conclusion: Our data suggest that eNOS gene polymorphisms may play an important role in the pathogenesis of vessel stenosis in Korean population.

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalClinica Chimica Acta
Issue number1-2
StatePublished - Mar 2005

Bibliographical note

Funding Information:
This work was supported in part by research grant from Ministry of Health and Welfare (HMP-00-P-21900-0017) to Drs. Inho Jo, Chol Shin and Hyun-Young Park. We thank Dr. Ho Kim for statistical analysis, Dr. Jin-Sung Lee for DNA preparation and invaluable suggestions and Ms. Jooyoung Lee and Ms. Sun Mi Lee for secretarial assistance.


  • Endothelial nitric oxide synthase gene
  • Korea
  • Polymorphism
  • Vessel stenosis


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