Endogenous sphingolipid metabolites related to the growth in Sphingomonas chungbukensis

Munkhtsatsral Burenjargal; Youn Sun Lee; Jae Myung Yoo; Young Chang Kim; Yong Moon Lee; Seikwan Oh; Yeo Pyo Yun; Jin Tae Hong; Youn Bok Chung; Dong Chul Moon; Hwan Soo Yoo

doi:10.1007/BF02977612

Endogenous sphingolipid metabolites related to the growth in Sphingomonas chungbukensis

Munkhtsatsral Burenjargal, Youn Sun Lee, Jae Myung Yoo, Young Chang Kim, Yong Moon Lee, Seikwan Oh, Yeo Pyo Yun, Jin Tae Hong, Youn Bok Chung, Dong Chul Moon, Hwan Soo Yoo

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Sphingolipids are present in animals, plants, fungi, yeasts and some bacteria. In mammalian cells sphingolipids act as lipid mediators for cell growth, differentiation, apoptosis and angiogenesis. In contrast, in bacteria the biological significance of sphingolipids has not been fully elucidated and sphingolipid metabolism has not been investigated. The aim of this study was to compare the pattern of sphingolipid metabolites in HIT-T15 β cells originating from hamster pancreas to that in the bacterial strain Sphingomonas chungbukensis DJ77, under various culture conditions. It was found that the concentration of cellular sphinganine (Sa) in S. chungbukensis was higher than that of sphingosine (So), while the level of cellular So in HIT-T15 cells was higher than that of Sa. Aeration and shaking during culture increased bacterial growth in S. chungbukensis, and the contents of So and Sa were also elevated. These results indicate that a de novo sphingolipid pathway appeared to be active in bacteria and that bacterial growth may be closely related to Sa levels.

Original language	English
Pages (from-to)	317-322
Number of pages	6
Journal	Archives of Pharmacal Research
Volume	30
Issue number	3
DOIs	https://doi.org/10.1007/BF02977612
State	Published - 31 Mar 2007

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (The Regional Research Universities Program/ Chungbuk BIT Research-Oriented University Consortium).

Keywords

Growth
HIT-T15β cells
HPLC
Sphingolipid
Sphingomonas chungbukensis

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10.1007/BF02977612

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title = "Endogenous sphingolipid metabolites related to the growth in Sphingomonas chungbukensis",

abstract = "Sphingolipids are present in animals, plants, fungi, yeasts and some bacteria. In mammalian cells sphingolipids act as lipid mediators for cell growth, differentiation, apoptosis and angiogenesis. In contrast, in bacteria the biological significance of sphingolipids has not been fully elucidated and sphingolipid metabolism has not been investigated. The aim of this study was to compare the pattern of sphingolipid metabolites in HIT-T15 β cells originating from hamster pancreas to that in the bacterial strain Sphingomonas chungbukensis DJ77, under various culture conditions. It was found that the concentration of cellular sphinganine (Sa) in S. chungbukensis was higher than that of sphingosine (So), while the level of cellular So in HIT-T15 cells was higher than that of Sa. Aeration and shaking during culture increased bacterial growth in S. chungbukensis, and the contents of So and Sa were also elevated. These results indicate that a de novo sphingolipid pathway appeared to be active in bacteria and that bacterial growth may be closely related to Sa levels.",

keywords = "Growth, HIT-T15β cells, HPLC, Sphingolipid, Sphingomonas chungbukensis",

author = "Munkhtsatsral Burenjargal and Lee, {Youn Sun} and Yoo, {Jae Myung} and Kim, {Young Chang} and Lee, {Yong Moon} and Seikwan Oh and Yun, {Yeo Pyo} and Hong, {Jin Tae} and Chung, {Youn Bok} and Moon, {Dong Chul} and Yoo, {Hwan Soo}",

note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (The Regional Research Universities Program/ Chungbuk BIT Research-Oriented University Consortium).",

year = "2007",

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doi = "10.1007/BF02977612",

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AU - Burenjargal, Munkhtsatsral

AU - Lee, Youn Sun

AU - Yoo, Jae Myung

AU - Kim, Young Chang

AU - Lee, Yong Moon

AU - Oh, Seikwan

AU - Yun, Yeo Pyo

AU - Hong, Jin Tae

AU - Chung, Youn Bok

AU - Moon, Dong Chul

AU - Yoo, Hwan Soo

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (The Regional Research Universities Program/ Chungbuk BIT Research-Oriented University Consortium).

PY - 2007/3/31

Y1 - 2007/3/31

N2 - Sphingolipids are present in animals, plants, fungi, yeasts and some bacteria. In mammalian cells sphingolipids act as lipid mediators for cell growth, differentiation, apoptosis and angiogenesis. In contrast, in bacteria the biological significance of sphingolipids has not been fully elucidated and sphingolipid metabolism has not been investigated. The aim of this study was to compare the pattern of sphingolipid metabolites in HIT-T15 β cells originating from hamster pancreas to that in the bacterial strain Sphingomonas chungbukensis DJ77, under various culture conditions. It was found that the concentration of cellular sphinganine (Sa) in S. chungbukensis was higher than that of sphingosine (So), while the level of cellular So in HIT-T15 cells was higher than that of Sa. Aeration and shaking during culture increased bacterial growth in S. chungbukensis, and the contents of So and Sa were also elevated. These results indicate that a de novo sphingolipid pathway appeared to be active in bacteria and that bacterial growth may be closely related to Sa levels.

AB - Sphingolipids are present in animals, plants, fungi, yeasts and some bacteria. In mammalian cells sphingolipids act as lipid mediators for cell growth, differentiation, apoptosis and angiogenesis. In contrast, in bacteria the biological significance of sphingolipids has not been fully elucidated and sphingolipid metabolism has not been investigated. The aim of this study was to compare the pattern of sphingolipid metabolites in HIT-T15 β cells originating from hamster pancreas to that in the bacterial strain Sphingomonas chungbukensis DJ77, under various culture conditions. It was found that the concentration of cellular sphinganine (Sa) in S. chungbukensis was higher than that of sphingosine (So), while the level of cellular So in HIT-T15 cells was higher than that of Sa. Aeration and shaking during culture increased bacterial growth in S. chungbukensis, and the contents of So and Sa were also elevated. These results indicate that a de novo sphingolipid pathway appeared to be active in bacteria and that bacterial growth may be closely related to Sa levels.

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KW - HIT-T15β cells

KW - HPLC

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KW - Sphingomonas chungbukensis

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Endogenous sphingolipid metabolites related to the growth in Sphingomonas chungbukensis

Abstract

Bibliographical note

Keywords

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