TY - JOUR
T1 - Emission detection of mercuric ions in aqueous media based-on dehybridization of DNA duplexes
AU - Oh, Byul Nim
AU - Wu, Qiong
AU - Cha, Mi Sun
AU - Kang, Hee Kyung
AU - Kim, Jin Ah
AU - Kim, Ka Young
AU - Rajkumar, Eswaran
AU - Kim, Jinheung
PY - 2011/9/20
Y1 - 2011/9/20
N2 - To quantify the presence of mercuric ions in aqueous solution, double-stranded DNA (dsDNA) of poly(dT) was employed using a light switch compound, Ru(phen)2(dppz)2+ (1) which is reported to intercalate into dsDNA of a right-handed B-form. Addition of mercuric ions induced the dehybridization of poly(dT)•poly(dA) duplexes to form a hairpin structure of poly(dT) at room temperature and the metal-to-ligand charge transfer emission derived from the intercalation of 1 was reduced due to the dehybridization of dsDNA. As the concentration of Hg2+ was increased, the emission of 1 progressively decreased. This label-free emission method had a detection limit of 0.2 nM. Other metal ions, such as K+, Ag +, Ca2+, Mg2+, Zn2+, Mn 2+, Co2+, Ni2+, Cu2+, Cd 2+, Cr3+, Fe3+, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the emission intensity of dsDNA.
AB - To quantify the presence of mercuric ions in aqueous solution, double-stranded DNA (dsDNA) of poly(dT) was employed using a light switch compound, Ru(phen)2(dppz)2+ (1) which is reported to intercalate into dsDNA of a right-handed B-form. Addition of mercuric ions induced the dehybridization of poly(dT)•poly(dA) duplexes to form a hairpin structure of poly(dT) at room temperature and the metal-to-ligand charge transfer emission derived from the intercalation of 1 was reduced due to the dehybridization of dsDNA. As the concentration of Hg2+ was increased, the emission of 1 progressively decreased. This label-free emission method had a detection limit of 0.2 nM. Other metal ions, such as K+, Ag +, Ca2+, Mg2+, Zn2+, Mn 2+, Co2+, Ni2+, Cu2+, Cd 2+, Cr3+, Fe3+, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the emission intensity of dsDNA.
KW - DNA intercalation
KW - Light switch Ru compound
KW - Mercuric ion sensing
KW - Metal-to-ligand transfer emission
KW - Mismatch detection
KW - Poly(dT)
UR - http://www.scopus.com/inward/record.url?scp=80053102802&partnerID=8YFLogxK
U2 - 10.5012/bkcs.2011.32.9.3223
DO - 10.5012/bkcs.2011.32.9.3223
M3 - Article
AN - SCOPUS:80053102802
SN - 0253-2964
VL - 32
SP - 3223
EP - 3228
JO - Bulletin of the Korean Chemical Society
JF - Bulletin of the Korean Chemical Society
IS - 9
ER -