Electricity generation coupled to oxidation of propionate in a microbial fuel cell

Jae Kyung Jang; In Seop Chang; Hwa Yeon Hwang; Yeng Fung Choo; Ji Young Lee; Kyung Suk Cho; Byung Hong Kim; Kenneth H. Nealson

doi:10.1007/s10529-009-0118-y

Electricity generation coupled to oxidation of propionate in a microbial fuel cell

Jae Kyung Jang, In Seop Chang, Hwa Yeon Hwang, Yeng Fung Choo, Ji Young Lee, Kyung Suk Cho, Byung Hong Kim, Kenneth H. Nealson

Department of Environmental Science & Engineering

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Propionate was used as fuel to enrich an electrochemically-active microbial consortium in a microbial fuel cell, and the bacterial consortium was analyzed by culture-independent methods including denaturing gradient gel electrophoresis (DGGE) of the 16S rDNA, and by fluorescent in situ hybridization (FISH). MFCs fed with propionate produced a current of 4.88 ± 0.1 mA stably on 100 mg propionate/l as COD within 3 weeks of the enrichment. When the MFCs were fed with H₂-saturated fuel containing propionate, the current dropped to 3.82 ± 0.07 mA. The maximum current generated was up to 8.8 mA when MFCs were fed with 200 mg propionate/l as COD. The DGGE of 16S rDNA showed that propionate-enriched MFCs have a different bacterial population from that enriched with acetate and from the inoculum used for enrichment. The major member (42%) of the consortium was an unidentified bacterium followed by γ, β, and δ-proteobacteria.

Original language	English
Pages (from-to)	79-85
Number of pages	7
Journal	Biotechnology Letters
Volume	32
Issue number	1
DOIs	https://doi.org/10.1007/s10529-009-0118-y
State	Published - Jan 2010

Bibliographical note

Funding Information:
Acknowledgements This study was supported by the Korea Research Foundation Grant funded by the Korean Government’’ (KRF-2006-214-D00081), in which main calculations were performed by using the supercomputing resource of the Korea Institute of Science and Technology Information (KISTI). This study was also partly supported by the Korea Science and Engineering Foundation (KOSEF) NRL program grant funded by the Korea government (MEST) (No. R0A-2008-000-20088-0).

Keywords

16S rDNA
Bacterial community
Electricity generation
Microbial fuel cell (MFC)
Propionate

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title = "Electricity generation coupled to oxidation of propionate in a microbial fuel cell",

abstract = "Propionate was used as fuel to enrich an electrochemically-active microbial consortium in a microbial fuel cell, and the bacterial consortium was analyzed by culture-independent methods including denaturing gradient gel electrophoresis (DGGE) of the 16S rDNA, and by fluorescent in situ hybridization (FISH). MFCs fed with propionate produced a current of 4.88 ± 0.1 mA stably on 100 mg propionate/l as COD within 3 weeks of the enrichment. When the MFCs were fed with H2-saturated fuel containing propionate, the current dropped to 3.82 ± 0.07 mA. The maximum current generated was up to 8.8 mA when MFCs were fed with 200 mg propionate/l as COD. The DGGE of 16S rDNA showed that propionate-enriched MFCs have a different bacterial population from that enriched with acetate and from the inoculum used for enrichment. The major member (42%) of the consortium was an unidentified bacterium followed by γ, β, and δ-proteobacteria.",

keywords = "16S rDNA, Bacterial community, Electricity generation, Microbial fuel cell (MFC), Propionate",

author = "Jang, {Jae Kyung} and Chang, {In Seop} and Hwang, {Hwa Yeon} and Choo, {Yeng Fung} and Lee, {Ji Young} and Cho, {Kyung Suk} and Kim, {Byung Hong} and Nealson, {Kenneth H.}",

note = "Funding Information: Acknowledgements This study was supported by the Korea Research Foundation Grant funded by the Korean Government{\textquoteright}{\textquoteright} (KRF-2006-214-D00081), in which main calculations were performed by using the supercomputing resource of the Korea Institute of Science and Technology Information (KISTI). This study was also partly supported by the Korea Science and Engineering Foundation (KOSEF) NRL program grant funded by the Korea government (MEST) (No. R0A-2008-000-20088-0).",

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doi = "10.1007/s10529-009-0118-y",

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AU - Jang, Jae Kyung

AU - Chang, In Seop

AU - Hwang, Hwa Yeon

AU - Choo, Yeng Fung

AU - Lee, Ji Young

AU - Cho, Kyung Suk

AU - Kim, Byung Hong

AU - Nealson, Kenneth H.

N1 - Funding Information: Acknowledgements This study was supported by the Korea Research Foundation Grant funded by the Korean Government’’ (KRF-2006-214-D00081), in which main calculations were performed by using the supercomputing resource of the Korea Institute of Science and Technology Information (KISTI). This study was also partly supported by the Korea Science and Engineering Foundation (KOSEF) NRL program grant funded by the Korea government (MEST) (No. R0A-2008-000-20088-0).

PY - 2010/1

Y1 - 2010/1

N2 - Propionate was used as fuel to enrich an electrochemically-active microbial consortium in a microbial fuel cell, and the bacterial consortium was analyzed by culture-independent methods including denaturing gradient gel electrophoresis (DGGE) of the 16S rDNA, and by fluorescent in situ hybridization (FISH). MFCs fed with propionate produced a current of 4.88 ± 0.1 mA stably on 100 mg propionate/l as COD within 3 weeks of the enrichment. When the MFCs were fed with H2-saturated fuel containing propionate, the current dropped to 3.82 ± 0.07 mA. The maximum current generated was up to 8.8 mA when MFCs were fed with 200 mg propionate/l as COD. The DGGE of 16S rDNA showed that propionate-enriched MFCs have a different bacterial population from that enriched with acetate and from the inoculum used for enrichment. The major member (42%) of the consortium was an unidentified bacterium followed by γ, β, and δ-proteobacteria.

AB - Propionate was used as fuel to enrich an electrochemically-active microbial consortium in a microbial fuel cell, and the bacterial consortium was analyzed by culture-independent methods including denaturing gradient gel electrophoresis (DGGE) of the 16S rDNA, and by fluorescent in situ hybridization (FISH). MFCs fed with propionate produced a current of 4.88 ± 0.1 mA stably on 100 mg propionate/l as COD within 3 weeks of the enrichment. When the MFCs were fed with H2-saturated fuel containing propionate, the current dropped to 3.82 ± 0.07 mA. The maximum current generated was up to 8.8 mA when MFCs were fed with 200 mg propionate/l as COD. The DGGE of 16S rDNA showed that propionate-enriched MFCs have a different bacterial population from that enriched with acetate and from the inoculum used for enrichment. The major member (42%) of the consortium was an unidentified bacterium followed by γ, β, and δ-proteobacteria.

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KW - Electricity generation

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Electricity generation coupled to oxidation of propionate in a microbial fuel cell

Abstract

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Keywords

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