Eicosapentaenoic acid increases lipolysis through up-regulation of the lipolytic gene expression and down-regulation of the adipogenic gene expression in 3T3-L1 adipocytes

Mak Soon Lee, In Sook Kwun, Yangha Kim

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

In this study, we investigated the lipolytic effects of eicosapentaenoic acid (EPA) in 3T3-L1 adipocytes. The differentiated 3T3-L1 adipocytes were treated in a serum-free medium with 300 μM of EPA for 3, 6, 12, and 24 h. In comparison with the control, intracellular lipid accumulation was significantly decreased by 24% at 24 h following the addition of EPA (P < 0.05). Under the same experimental conditions, there was an increase of glycerol and free fatty acids (FFAs). The mRNA level of carnitine palmitoyltransferase I-a, a component of the fatty-acid shuttle system involved in the mitochondrial oxidation of long-chain fatty acids, was also significantly elevated by EPA (P < 0.05). However, the expression of peroxisome proliferator-activated receptor-γ and acetyl-CoA carboxylase (ACC), which are involved in adipogenesis, was significantly down-regulated by EPA (P < 0.05). These results suggest that EPA may modulate lipid metabolism by stimulation of lipolysis, which was associated with induction of lipolytic gene expression and suppression of adipogenic gene expression in 3T3-L1 adipocytes.

Original languageEnglish
Pages (from-to)327-330
Number of pages4
JournalGenes and Nutrition
Volume2
Issue number4
DOIs
StatePublished - Feb 2008

Keywords

  • 3T3-adipocytes
  • Acetyl-CoA carboxylase
  • Carnitine palmitoyltransferase I-α
  • EPA
  • Free fatty acid
  • Gene expression
  • Glycerol
  • Lipolysis
  • Peroxisome proliferator-activated receptor-γ

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