Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph

S. H. Ha, T. H. Park

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Silkworm hemolymph when added at 5% (v/v) to medium increased the production of recombinant β-galactosidase in Spodoptera frugipera/Autographa californica nuclear polyhedrosis virus (AcNPV) system by 4.5-fold. Silkworm hemolymph also increased the host cell longevity by two-fold. Viability of the host cell was maintained at a constant level for 6 days after baculovirus infection in the medium containing 5% silkworm hemolymph, while host cells began to die 3 days after infection in the medium without hemolymph.

Original languageEnglish
Pages (from-to)1087-1091
Number of pages5
JournalBiotechnology Letters
Volume19
Issue number11
DOIs
StatePublished - 1997

Bibliographical note

Funding Information:
This work was supported by ‘95 biochemical engineering supporting program (the Korean Ministry of Education) and Sericulture & Insect Research Institute.

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