Efficient production of gamma-aminobutyric acid using Escherichia coli by co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter

Van Dung Pham, Sivachandiran Somasundaram, Seung Hwan Lee, Si Jae Park, Soon Ho Hong

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Gamma-aminobutyric acid (GABA) is an important bio-product, which is used in pharmaceutical formulations, nutritional supplements, and biopolymer monomer. The traditional GABA process involves the decarboxylation of glutamate. However, the direct production of GABA from glucose is a more efficient process. To construct the recombinant strains of Escherichia coli, a novel synthetic scaffold was introduced. By carrying out the co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter, we redirected the TCA cycle flux to GABA pathway. The genetically engineered E. coli strain produced 1.08 g/L of GABA from 10 g/L of initial glucose. Thus, with the introduction of a synthetic scaffold, we increased GABA production by 2.2-fold. The final GABA concentration was increased by 21.8 % by inactivating competing pathways.

Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalJournal of Industrial Microbiology and Biotechnology
Volume43
Issue number1
DOIs
StatePublished - 1 Jan 2016

Keywords

  • Co-localization
  • GABA
  • Protein scaffold
  • Synthetic biology

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