TY - JOUR
T1 - Efficient bioconversion of sugarcane bagasse into polyhydroxybutyrate (PHB) by Lysinibacillus sp. and its characterization
AU - Saratale, Rijuta Ganesh
AU - Cho, Si Kyung
AU - Saratale, Ganesh Dattatraya
AU - Ghodake, Gajanan S.
AU - Bharagava, Ram Naresh
AU - Kim, Dong Su
AU - Nair, Supriya
AU - Shin, Han Seung
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/3
Y1 - 2021/3
N2 - In this study, Lysinibacillus sp. RGS was evaluated to synthesize polyhydroxybutyrate (PHB) from a broad range of pure carbon sources and residual sugars of chemically pretreated sugarcane bagasse (SCB) hydrolysates. Effects of supplementation of nutrients and various experimental variables to enhance PHB accumulation were investigated. Results of optimized parameters were identified as 48 h, 37 °C, pH 7; inoculums concentration (2.5% v/v) and shaking condition (100 rpm). Growth kinetics and bioprocess parameters of Lysinibacillus sp. using SCB hydrolysates with corn steep liquor (2%) accounted for the maximum cell growth (8.65 g/L) and PHA accumulation (61.5%) with PHB titer of (5.31 g/L) under optimal conditions. The produced biopolymer was studied by Fourier Transform Infrared (FTIR) spectroscopy and the results revealed the obtained to be PHB. Thus Lysinibaciluus sp. exhibits high potential in industrial scale manufacture of PHB using SCB as an inexpensive substrate.
AB - In this study, Lysinibacillus sp. RGS was evaluated to synthesize polyhydroxybutyrate (PHB) from a broad range of pure carbon sources and residual sugars of chemically pretreated sugarcane bagasse (SCB) hydrolysates. Effects of supplementation of nutrients and various experimental variables to enhance PHB accumulation were investigated. Results of optimized parameters were identified as 48 h, 37 °C, pH 7; inoculums concentration (2.5% v/v) and shaking condition (100 rpm). Growth kinetics and bioprocess parameters of Lysinibacillus sp. using SCB hydrolysates with corn steep liquor (2%) accounted for the maximum cell growth (8.65 g/L) and PHA accumulation (61.5%) with PHB titer of (5.31 g/L) under optimal conditions. The produced biopolymer was studied by Fourier Transform Infrared (FTIR) spectroscopy and the results revealed the obtained to be PHB. Thus Lysinibaciluus sp. exhibits high potential in industrial scale manufacture of PHB using SCB as an inexpensive substrate.
KW - Corn steep liquor
KW - Lysinibacillus sp. RGS
KW - Peracetic acid pretreatment
KW - Poly(3-hydroxybutyrate)
KW - Saccharification yield
KW - Sugarcane bagasse
UR - http://www.scopus.com/inward/record.url?scp=85099461616&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2021.124673
DO - 10.1016/j.biortech.2021.124673
M3 - Article
C2 - 33445010
AN - SCOPUS:85099461616
SN - 0960-8524
VL - 324
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 124673
ER -
