Effects of synthetic pseudoceramides on sphingosine kinase activity in F9-12 cells

You Xun Jin, Kyong Oh Shin, Myung Yong Park, Shin Hee Lee, Byeong Deog Park, Seikwan Oh, Hwan Soo Yoo, Yong Moon Lee

Research output: Contribution to journalArticlepeer-review


Sphingosine kinase (SPHK) has a central role to control cell death and cell proliferation, which is suggested as a sphingolipid rheostat by regulating the levels between ceramide and sphingosine 1-phosphate (S1P). Therefore, physiological regulators of SPHK will be a good candidate to develop a new targeted drug. For this purpose, a series of synthetic pseudoceramides were tested by SPHK assay either cell-based or cell-free system. K10PC-5 strongly inhibited SPHK, while K6PC-5 activated SPHK in cell-free system. Specifically, K6PC-5 activated SPHK under the co-treatment with 50 μM dimethylsphingosine (DMS), a SPHK inhibitor. Collectively, we developed a simple SPHK assay system to find SPHK regulatory pseudoceramide compounds, K10PC-5 and K6PC-5 which may be useful to cancer treatment or immune regulation like FTY720, a synthetic sphingolipid mimetic compound.

Original languageEnglish
Pages (from-to)134-139
Number of pages6
JournalBiomolecules and Therapeutics
Issue number1
StatePublished - Jan 2011


  • Assay system
  • F9 cells
  • Pseudoceramide
  • Sphingosine kinase


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