Effects of protostane-type triterpenoids on the 5-HT3A receptor-mediated ion current in Xenopus oocytes

Jun Ho Lee; Yoo Jin Lee; Seok Woo Kang; Yangseok Kim; Minkyu Shin; Moochang Hong; Eun Kyoung Seo; Sung Hoon Kim; Seung Yeol Nah; Hyunsu Bae

doi:10.1016/j.brainres.2010.03.041

Effects of protostane-type triterpenoids on the 5-HT₃A receptor-mediated ion current in Xenopus oocytes

Jun Ho Lee
, Yoo Jin Lee
, Seok Woo Kang
, Yangseok Kim
, Minkyu Shin
, Moochang Hong
, Eun Kyoung Seo
, Sung Hoon Kim
, Seung Yeol Nah
, Hyunsu Bae

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Alisol derivatives are unique protostane-type triterpenoid compounds that are isolated from Alismatis rhizoma, which is a well-known traditional medicine in East Asia. In the present study, we investigated the effects of protostane-type triterpenoids (AA, Alisol A; AB, Alisol B; AB-ac, Alisol B 23-acetate; AC-ac, Alisol C 23-aceteate) on 5-HT-induced currents mediated by the human 5-HT₃A receptor expressed in Xenopus laevis oocytes. Co-treatment with triterpenoids regulated the 5-HT-induced inward peak current in a concentration-dependent and reversible manner. In addition, regulation of I_5-HT by triterpenoids occurred in a non-competitive manner. Taken together, these results indicate that triterpenoids may regulate the 5-HT₃A receptors that are expressed in Xenopus oocytes. Furthermore, this regulation of the ligand-gated ion channel activity by triterpenoids may be one of the pharmacological actions of Alismatis rhizoma.

Original language	English
Pages (from-to)	20-27
Number of pages	8
Journal	Brain Research
Volume	1331
DOIs	https://doi.org/10.1016/j.brainres.2010.03.041
State	Published - 17 May 2010

Bibliographical note

Funding Information:
This work was supported by a grant from the Kyung-Hee University Post-Doctoral fellowship in 2009 ( KHU-20090503 )

Keywords

5-HTA receptor
Triterpenoid
Two-electrode voltage clamp

Access to Document

10.1016/j.brainres.2010.03.041

Cite this

@article{fa5bb62c2a0340968dce68848422304e,

title = "Effects of protostane-type triterpenoids on the 5-HT3A receptor-mediated ion current in Xenopus oocytes",

abstract = "Alisol derivatives are unique protostane-type triterpenoid compounds that are isolated from Alismatis rhizoma, which is a well-known traditional medicine in East Asia. In the present study, we investigated the effects of protostane-type triterpenoids (AA, Alisol A; AB, Alisol B; AB-ac, Alisol B 23-acetate; AC-ac, Alisol C 23-aceteate) on 5-HT-induced currents mediated by the human 5-HT3A receptor expressed in Xenopus laevis oocytes. Co-treatment with triterpenoids regulated the 5-HT-induced inward peak current in a concentration-dependent and reversible manner. In addition, regulation of I5-HT by triterpenoids occurred in a non-competitive manner. Taken together, these results indicate that triterpenoids may regulate the 5-HT3A receptors that are expressed in Xenopus oocytes. Furthermore, this regulation of the ligand-gated ion channel activity by triterpenoids may be one of the pharmacological actions of Alismatis rhizoma.",

keywords = "5-HTA receptor, Triterpenoid, Two-electrode voltage clamp",

author = "Lee, \{Jun Ho\} and Lee, \{Yoo Jin\} and Kang, \{Seok Woo\} and Yangseok Kim and Minkyu Shin and Moochang Hong and Seo, \{Eun Kyoung\} and Kim, \{Sung Hoon\} and Nah, \{Seung Yeol\} and Hyunsu Bae",

note = "Funding Information: This work was supported by a grant from the Kyung-Hee University Post-Doctoral fellowship in 2009 ( KHU-20090503 ) ",

year = "2010",

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doi = "10.1016/j.brainres.2010.03.041",

language = "English",

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journal = "Brain Research",

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T1 - Effects of protostane-type triterpenoids on the 5-HT3A receptor-mediated ion current in Xenopus oocytes

AU - Lee, Jun Ho

AU - Lee, Yoo Jin

AU - Kang, Seok Woo

AU - Kim, Yangseok

AU - Shin, Minkyu

AU - Hong, Moochang

AU - Seo, Eun Kyoung

AU - Kim, Sung Hoon

AU - Nah, Seung Yeol

AU - Bae, Hyunsu

N1 - Funding Information: This work was supported by a grant from the Kyung-Hee University Post-Doctoral fellowship in 2009 ( KHU-20090503 )

PY - 2010/5/17

Y1 - 2010/5/17

N2 - Alisol derivatives are unique protostane-type triterpenoid compounds that are isolated from Alismatis rhizoma, which is a well-known traditional medicine in East Asia. In the present study, we investigated the effects of protostane-type triterpenoids (AA, Alisol A; AB, Alisol B; AB-ac, Alisol B 23-acetate; AC-ac, Alisol C 23-aceteate) on 5-HT-induced currents mediated by the human 5-HT3A receptor expressed in Xenopus laevis oocytes. Co-treatment with triterpenoids regulated the 5-HT-induced inward peak current in a concentration-dependent and reversible manner. In addition, regulation of I5-HT by triterpenoids occurred in a non-competitive manner. Taken together, these results indicate that triterpenoids may regulate the 5-HT3A receptors that are expressed in Xenopus oocytes. Furthermore, this regulation of the ligand-gated ion channel activity by triterpenoids may be one of the pharmacological actions of Alismatis rhizoma.

AB - Alisol derivatives are unique protostane-type triterpenoid compounds that are isolated from Alismatis rhizoma, which is a well-known traditional medicine in East Asia. In the present study, we investigated the effects of protostane-type triterpenoids (AA, Alisol A; AB, Alisol B; AB-ac, Alisol B 23-acetate; AC-ac, Alisol C 23-aceteate) on 5-HT-induced currents mediated by the human 5-HT3A receptor expressed in Xenopus laevis oocytes. Co-treatment with triterpenoids regulated the 5-HT-induced inward peak current in a concentration-dependent and reversible manner. In addition, regulation of I5-HT by triterpenoids occurred in a non-competitive manner. Taken together, these results indicate that triterpenoids may regulate the 5-HT3A receptors that are expressed in Xenopus oocytes. Furthermore, this regulation of the ligand-gated ion channel activity by triterpenoids may be one of the pharmacological actions of Alismatis rhizoma.

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KW - Triterpenoid

KW - Two-electrode voltage clamp

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